黄芩苷的解热作用及对细胞因子的影响

Efffect of baicalin on antipyresis and influence on cytokine

目的:观察黄芩苷对酵母性发热大鼠的解热作用及对致热性细胞因子的影响,以探讨其对酵母性发热大鼠解热的可能机制。方法:sc酵母(2 g.kg-1)复制酵母性大鼠发热模型;大鼠随机分为对照组、模型组、黄芩苷高、中、低剂量组,观察药后各组体温变化;采用双抗体夹心ELSIA法测定发热高峰大鼠血浆、下丘脑及脑脊液中白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的含量。并将黄芩苷3个剂量组的温度变化率与IL-6,IL-1,βTNF-α含量变化率分别作相关性分析。结果:黄芩苷高剂量组较其他组能明显降低发热大鼠的体温,并且能降低大鼠血清、下丘脑、脑脊液中的IL-6,IL-1β,TNF-α含量。相关分析表明黄芩苷对大鼠的体温变化率与IL-1β在血清、下丘脑、脑脊液的变化率呈正相关(r=0.873,P<0.05),与TNF-α的含量变化率也呈正相关(r=0.862,P<0.01)。结论:黄芩苷有明显的解热作用,其作用机制可能主要与减少TNF-α和IL-1β的含量有关。

Objective： To study the antipyretic effect of baicalin in inhibiting yeast-induced fever in rats and the influence on inflammatory cytokine,then explore the possible mechanism of baicalin in inhibiting yeast-induced fever in rats.Method： Rat modles of pyrexia were established by subcutaneous injection of yeast（2 g·kg-1）;the rats of were divided into the normal control,model,baicalin high,medium and low-dose group and the effect of baicalin on the changes of the rats′ temperature were observed.Dual antibody ELISA method was used to test the changes of IL-6,IL-1β and TNF-α contents in in serum,hypothalamus and cerebral spinal fluid（CSF）.Then analyze the correlation between the inhibition ratio of temperature heighten on three different dose of baicalin and the inhibition ratio of the contents heighten on IL-6,IL-1β and TNF-α.Result： The high dose of baicalin significantly inhibited the yeast-induced fever of rats,and decresesed IL-6,IL-1β and TNF-α contents in serum,hypothalamus and CSF.The inhibition ratio of temperature heighten of baicalin had direct correlation with the inhibition ratio of the heighten on IL-1β content in serum,hypothalamus and CSF（r=0.873,P0.05）,also dose TNF-α（r=0.862,P0.01）.Conclusion： Baicalin may have obvious antipyretic effect by decreasing the increasing contents of IL-1β and TNF-α in rats.