5α还原酶抑制剂对老年大鼠5α还原酶mRNA表达的影响

Prostatic expression of rat 5alpha-reductase mRNA during 5alpha-reductase inhibitor used

目的 探讨5α还原酶抑制剂对老年大鼠5α还原酶mRNA表达的影响.方法 取50只健康的20周龄雄性SPF级SD大鼠,分为依立雄胺组（20只,喂饲依立雄胺3mg·kg^-1·d^-1）、非那雄胺组（20只,喂饲1 mg·kg^-1·d^-1）、正常对照组（10只,喂饲0.9%氯化钠溶液）.检测所有大鼠前列腺组织中Srd5a1、Srd5a2基因及bcl-2的表达情况,并作组间比较分析.结果 依立雄胺组、非那雄胺组、正常对照组Srd5a1的表达相对量分别为1.46±1.98、2.18±2.53、1.97±2.43,Srd5a2的表达相对量分别为1.31±0.37、1.29±0.47、1.05±0.36,bcl-2的表达分别为148±42、152±59、216±52;3组间Srd5a1、Srd5a2基因的表达并无明显差异（P〉0.05）,而依立雄胺组及非那雄胺组bcl-2的表达均明显低于正常对照组（均P〈0.05）,但依立雄胺组与非那雄胺组并无明显差异（P〉0.05）.结论 5α还原酶抑制剂可以减少凋亡抑制因子bcl-2的表达,从而促进前列腺增生组织细胞凋亡的发生,但可能并不能影响5α还原酶mRNA的表达.

Objective To identify the effection of two type 5alpha-reductase inhibitor on the prostatic expression of old male rat 5alpha-reductase mRNA. Methods 50 old male rats were divided into three groups as group epristeride, finasteride and control, wich were administrated orally by epristeride （20, 3mg·kg^-1·d^-1）, finasteride （20, 3mg·kg^-1·d^-1） or normal saline（10） respectively.The expressions of two type 5alpha-reductase mRNA and bcl-2 were detected and analyzed. Results The relative amount of expression of Srd5al of the epristeride, finasteride and control group were 1.46 ± 1.98,2.18 ± 2.53,1.97 ± 2.43, Srd5a2 were 1.31 ± 0.37,1.29 ± 0.47,1.05 ± 0.36, and the bcl-2 were 1.31 ± 0.37,1.29 ± 0.47,1.05 ± 0.36. There were no differences of the expressions of Srd5al ,Srd5a2 between any two groups （P 〉 0.05 ）, but the expressions of bcl-2 of epristeride and finasteride group were both significantly lower than that of the control group （P 〈 0.05 }. There were no difference of the expression of bcl-2 between epristeride group and finasteride group （P 〉 0.05）. Conclusion 5alpha-reductase inhibitor can induce the aptosis of prostate cell by reduing the expression of bcl-2,but it seems no effect on the expression of two types of 5alpha-reductase mRNA.