摘要
目的 探讨HER-2蛋白表达和基因扩增与乳腺癌淋巴结转移和雌激素受体（ER）、孕激素受体（PR）的关系.方法 采用免疫组化SP法和荧光原位杂交法检测64例乳腺癌新鲜标本中的HER-2蛋白表达和基因扩增情况,并结合ER、PR及淋巴结转移情况对比分析.结果 64例中HER-2蛋白表达强阳性15例,阳性7例,弱阳性3例,阴性39例,其中基因过表达（免疫组化检测强阳性或阳性）率为37.5%;基因扩增20例.无扩增44例.扩增率31.3%.免疫组化与荧光原位杂交检测符合率为90.9%（P〈0.05）.ER、PR表达情况与HER-2蛋白表达和基因扩增呈负相关（r=-0.36、-0.35,P〈0.05）,ER和PR均阴性者HER-2蛋白表达率（60.0%）及基因扩增率（55.0%）明显高于ER和（或）PR阳性者（分别为22.7%、20.5%）.差异均有统计学意义（×2=8.47、7.64,均P〈0.05）.淋巴结转移情况与HER-2蛋白表达及基因扩增无关（r=0.16、0.22,P〉0.05）.结论 HER-2蛋白表达和基因扩增提示乳腺癌预后不良,HER-2及ER、PR在乳腺癌的治疗中有着重要作用,免疫组化可作为检测HER-2基因状态的首选方法 ,但对于HER-2＋＋者需进一步行荧光原位杂交检测.HER-2蛋白表达和基因扩增与淋巴结转移无关.
Objective To study the amplification and expression of HER-2 gene and proteinum in breast cancer and to investigate the relationship between HER-2 and proteinum and ER,PR and lymph node metastasis. Methods Apply fluorescence in situ hybridization （FISH） and immunohistochemistry （IHC） method to detect the amplification and expression of HER-2 gene and proteinum and the expression of ER,PR and lymph node metastasis in 64 case of breast cancer. Results HER-2 overexpression was 22, with （＋＋＋） 15 cases and （＋＋） 7 cases. There are 20 cases to find HER-2 gene amplification,the positive rate was 31.35% ,the coincidence of HER-2 overexpression and HER-2 gene amplification was 90.9% （P〈0.05）,The expression of HER-2 had negative correlation with the expression ER,PR （r=-0.36,-0.35, P〈0.05）, HER-2 gene and proteinum positive expression was significant higher in PR and ER negative group than in PR and （or） ER positive group （IHC 60.0% vs 22.7%, P〈0.05, FISH 55.0% vs 20.5%,X^2=8.47,7.64, P〈0.05）. HER-2 gene amplification had no statistical significance with lymph node metastasis （P〉0.05）. Conclusion The amplification and overexpression of HER-2 gene and proteinum foreshow breast cancer was insensitive for some treatment plan and unfavourable prognosis. ER,PR and HER-2 might play a important role on the occurrence and development in breast cancer.
出处
《浙江医学》
CAS
2010年第2期166-168,172,共4页
Zhejiang Medical Journal
