摘要
目的 探讨子宫内膜异位症（内异症）患者腹腔液白细胞介素6（IL-6）及其膜结合受体（mIL-6R）、可溶性受体（sIL-6R）在内异症发病机制中的意义.方法 47例经腹腔镜手术的内异症患者根据r-AFS分期分为A组（Ⅰ～Ⅱ期：19例）和B组Ⅲ～Ⅳ期：28例）,以22例同期腹腔镜手术的卵巢良性囊肿、子宫畸形患者为对照组,CD126-PE荧光抗体标记mIL-6R,流式细胞法检测腹腔液中CD126＋细胞的表达;同时应用酶联免疫吸附试验（ELISA法）检测腹腔液中IL-6及sIL-6R的含量.结果 A组和B组腹腔液中IL-6及mIL-6R的表达显著高于对照组（均P〈0.05）,两者的升高保持一致性,但无明显的直线相关关系,而B组患者腹腔液中sIL-6R含量显著高于A组和对照组（均P〈0.05）.结论 内异症患者腹腔液中升高的IL-6可能首先通过与同样表达增强的mIL-6R结合而影响疾病发生与发展;同时内异症患者腹腔液sIL-6R含量显著升高,协同参与了内异症的发展,并且可能与疾病的严重程度相关;而内异症患者腹腔液中大量mIL-6R的水解脱落可能是显著升高的sIL-6R主要的产生来源.
Objective To determine interleukin 6 （IL-6）, membrane interleukin 6 receptor（ mlL-6R ）and soluble interleukin 6 receptor （slL-6R） in peritoneal fluid of women with endometriosis. Methods Forty seven woman with endometriosis confirmed by laparoscopy and histological examination were enrolled in the study; and according to the revised American Fertility Society （rAFS） classification 19 cases were classified as stage I-II （group A） and 28 cases as stage Ill-IV （group B）. Twenty two women with benign ovarian cystic tumors, uterine malformation were taken as the control group, mlL-6R in peritoneal fluid samples was detected by flow cytometry with CD126-PE-labeled monoclonal antibody; double antibody sandwiched ELISA kits were used to detect the IL-6 and sIL-6R levels in peritoneal fluid. Results The IL-6 and mlL-6R levels in group A and B were higher than those in control group （P〈 0.05）; and slL-6R levels in group B were higher than those in control group and group A （P〈 0.05）. Conclusion The results suggest that the hinging of IL-6 to mlL-6R may be involved in the pathogenesis and progress of endometriosis; and the increased slL-6R may be associated with the severity of the disease.
出处
《浙江医学》
CAS
2010年第2期169-172,共4页
Zhejiang Medical Journal
基金
浙江省卫生厅科学研究基金项目（2006A06）
