川芎嗪对兔肾缺血再灌注损伤时血栓素A2/前列环素失衡的调控作用

Regulating effect of ligustrazine on thromboxane/prostacyclin during renal ischemia reperfusion injury in rabbts

目的 观察兔肾缺血再灌注损伤时血栓素A2（TXA2）/前列环素（PGl2）的变化,探讨JII芎嗪的保护作用及其机制.方法 将30只日本大耳白兔随机分为3组：假手术对照组（对照组）、缺血再灌注组（再灌注组）、缺血再灌注＋川芎嗪注射液组（川芎嗪组）,同时予再灌注组和川芎嗪组复制兔肾缺血再灌注损伤动物模型,并在缺血前、缺血1h、再灌注5 h时分别经颈总动脉抽血检测TXA2和PGl2降解后的代谢产物TXB2和6-酮-前列腺素F1α（6-keto-PGF1α）含量,计算其比值;实验结束时检测肾组织TXB2和6-keto-PGF1α含量与比值,并观察超微结构变化,作对比分析.结果 再灌注组缺血后各时点的血浆TXB2水平均较对照组明显增高（均P〈0.01）,川芎嗪组缺血后各时点的血浆TXB2含量均较再灌注组和对照组明显降低（均P〈0.01）;川芎嗪组血浆和肾组织TXB2/6-keto-PGF1α水平均较再灌注组明显降低（均P〈0.01）;再灌注组肾小球毛细血管和内皮细胞及近曲小管上皮细胞超微结构均严重受损,而川芎嗪组虽大部分肾组织上述部位均存在不同程度损伤性改变,但均较再灌注组明显减轻.结论 川芎嗪可抑制血小板释放TXA2,调控TXA2/PGl2的平衡,遏制无复流现象,对肾缺血再灌注损伤具有防治作用.

Objective To investigate the regulating effects of ligustrazine on thromboxane/prostacyclin in renal ischemia reperfusion injury of rabbits. Methods Thirty rabbits were randomly divided into three groups （n= 10 in each）： sham operation group （S group）, ischemia reperfusion injury group （IR group） and ischemia reperfusion injury plus ligustrazine injection group （LZ group）. The blood samples were taken from common carotid artery at pre-ischemia, lh after ischernia, 5h after reperfusion; plasma contents of thromboxane B2 （TXB2） and 6-keto-prostaglandin Flo （6-keto-PGF1a） were tested. The kidney tissue samples were collected at the end of experiment, the ultrastructural changes were examined under electronic microscope （EM） and the contents of TXB2 and 6-keto-PGF1a in renal tissue were assayed. Results The plasma content of TXB2 of IR group was significant higher than that in S group at all time points （all P〈0.01）. The plasma content of TXB2 in LZ group was decreased significantly compared with IR group and with S group at all time points during ischemia-reperfusion （P〈0.01）. The TXB2/6-keto-PGF1a contents in plasma and kidney tissue of LZ group were significant lower compared with IR group （all P〈0.01）. EM examination showed that the glomerular capillary, endotheliocytes and epithelial cells of proximal convoluted tubule in IR group presented marked abnormalities. Renal tissue ultrastructural injuries in LZ group were significantly alleviated as compared to IR group. Conclusion Ligustrazine can inhibit platelet to release TXA2, regulate TXA2/PGI2 balance and restrain no-reflow phenomenon, therefore to prevent kidney from ischemia reperfusion injury.