2-甲氧基雌二醇对骨髓瘤细胞作用机制的实验研究

Effect of 2-methoxyestradiol on proliferation and apoptosis of multiple myeloma cells

目的 探讨2-甲氧基雌二醇（2-ME）对骨髓瘤细胞作用的机制.方法 对1例复发性和难治性骨髓瘤细胞进行分离、培养,予0、1、2、4、8和16 μmol/L的2-ME对骨髓瘤细胞处理48h（以0 μmol/L的2-ME作为对照组）.后采用酶联免疫法检测培养上清中的血管内皮细胞生长因子（VEGF）和IL-6的表达水平,同时采用免疫组化法检测c-myc和bcl-2蛋白阳性表达的骨髓瘤细胞.结果 随着2-ME浓度的增加,培养上清中VEGF和IL-6的表达水平均较对照组明显降低（均P〈0.01）,且骨髓瘤细胞中c-myc和bcl-2蛋白表达阳性率均较对照组明显降低（均P〈0.01）.结论 2-ME对难治性骨髓瘤患者骨髓瘤细胞具有抑制VEGF、IL-6分泌和C-myc、bcl-2蛋白表达水平的作用.

Objective TO investigate the effect of 2-methoxyestradiol （2-ME） on multiple myeloma cells. Methods Multiple myeloma cells were separated from a relapsed and refractory patient. The cells were incubated with 2-methoxyestradiol （0, 1, 2, 4, 8 and 16 p mol/L） for 48h, and then the contents of vascular endothelial growth factor （VEGF） and interleukin-6 （IL-6） in the supematant were detected by enzyme linked ）mmunosorbent assay （ELISA）. The expressions of c-myc and bcl-2 proteins in myeloma cells were determined by immunohistochemisry. Results Compared with the controls, the contents of VEGF and IL-6 in the supernatant were decreased, the expressions of c-myc and bcl-2 proteins in myeloma cells were reduced, with 2-ME concentration increased and exposure time prolonged （both P〈0.01）. Conclusion 2-ME can decrease VEGF and IL-6, and down-regulate c-myc and bcl-2 protein expressions.