酵母蛋白自溶提取条件及氨基酸测定

Autolysis extraction of yeast protein and analysis of amino acids

本文研究了用废啤酒酵母为原料，采用自溶法提取酵母中的蛋白。发现添加质壁分离剂的量及自溶时间与提取率有显著关系。试验表明：质壁分离剂占体积比的7％，温度为55±1℃，时间为3h自溶效果最佳，可使酵母菌体中蛋白质的提取率达到12．2％，且氨基酸组成符合人体需要，特别是第一限制性氨基酸──赖氨酸含量较高。

The yeast protein was prepared from beer yeast by uslng autolysis. The results show that after 3h the autolytic effect could be the best when plasmolyticum was 7% under the temperature of 55℃ The extractive efficiency of protein could be 12. 2 %. The compositions of amino acids could meet the demands of human beings.