腺病毒介导的NDRG2基因对前列腺癌细胞株DU145的影响

Adenovirus-mediated NDRG2 gene expression in prostate cancer cell line DU145

目的:探讨腺病毒介导的N-myc下游调节基因2(NDRG2)基因对前列腺癌细胞株DU145增殖抑制及诱导其凋亡的作用。方法:以携带人NDRG2基因的腺病毒载体转染体外培养的前列腺癌细胞株DU145。采用Western blot检测目的基因的表达,通过细胞生长实验、平板克隆实验检测NDRG2对DU145细胞增殖能力的影响。流式细胞仪检测转染前后细胞凋亡的情况;光镜观察细胞形态学的改变。结果:DU145细胞经腺病毒转染后Western blot检测有NDRG2蛋白(40kD)特异表达。MTT比色及平板克隆实验结果显示NDRG2对DU145细胞生长有明显抑制作用(P<0.05)。流式细胞检测结果显示Ad-NDRG2组凋亡率明显高于对照组及Ad-LacZ组,差异有显著性(P<0.05)。与对照组及Ad-LacZ组比较,光镜下观察可见Ad-NDRG2转染的细胞生长状态明显变差,细胞变圆,边缘模糊。结论:通过腺病毒载体使细胞表达NDRG2基因,可以明显抑制DU145细胞的生长和增殖,并可诱导细胞凋亡。

Objective To investigate the effect of NDRG2 gene over-expression on the cell proliferation and apoptosis of prostate cancer DU145 cell. Methods Recombinant adenovirus was used to infected prostate cancer cell line DU145 in vitro. NDRG2 protein expression in cells infected with Ad-NDRG2 or Ad-LaeZ was detected using Westernblot assay. MTT and colony formation assays were performed to determine cell proliferation. Cell apoptosis was detected by flow cytometry. Cells morphology was observed under the light microscope. Results After transfected by adenovirus,it had been verified that the NDRG2 protein over-expression was identified cells infected with recombinant adenoviruses. NDRG2 inhibited DU145 cell growth and proliferation. The results of MTT and colony formation assay showed that NDRG2 could significantly inhibit DU145 cell proliferation （P 〈 0.05）. The apoptotic rate of cells infected with Ad-NDRG2 was significantly higher than that in control group and Ad-LacZ group（P 〈 0.05）. Cells in the Ad-NDRG2 group became round and were sicker than those in other two groups. Conclusion Recombinant adenovirus-mediated NDRG2 expression could inhibit DU145 cell growth and proliferation, and could enhance DU145 cell apoptosis.