艾司西酞普兰对慢性应激小鼠行为的影响

The effect of acute or chronic administration of escitalopram on behavior of chronic-stressed mice

目的观察急性或慢性给予艾司西酞普兰（Escitalopram，ESC）对慢性不可预知温和应激模型（Chronicunpredictablemildstress，CUMS）小鼠行为的影响。方法给予小鼠慢性应激8周，每2周进行行为学测试实验，同时急性或慢性给予艾司西酞普兰灌胃，比较急慢性给药的药效差异。结果（1）自应激第2周起，5个应激组体质量较对照组[（18．5±1．1）g]轻（P〈0．01），第8周时慢性给药组体质量[（18．5±0．6）g]较其他应激组重（P〈0．01）。（2）第2，4周，与对照组比较，5个应激组运动距离、站立次数及中央活动路程增加（P〈0．05），而第6，8周减低（P〈0．05）；第8周慢性给药组上述指标[（57．4±11．0）m（74．2±6．1）次（12．0±3．0）m]高于其他应激组（P〈0．05）。（3）各应激组糖水偏好值在第6，8周低于对照组（P〈0．05），第8周慢性给药组[（79．0±2．7）％]高于其他应激组（P〈0．05）。（4）各应激组在第4，6，8周强迫游泳不动时间比对照组长（P〈0．05），第8周慢性给药组不动时间[（124．70±8．00）S]比其他应激组短（P〈0．05）。（5）急性给药组小鼠的各项行为学指标与单纯的慢性应激组比较，差异无统计学意义（P〉0．05）。结论慢性给予应激模型小鼠艾司西酞普兰，显现出抗抑郁作用，而急性给药无抗抑郁效果。

Objective To investigate the effect of acute or chronic administration of escitalopram （ ESC ） on behavior of mice, which suffered from chronic unpredictable mild stress（CUMS）. Methods Miee were exposured to CUMS for 8 ,ueeks ,then the behavior of mice were in, Jestigated every 2 weeks, and the effect of acute and chronic administration of escitalopram on behavior was compared. Results （ 1 ） The body weight of all stressed mice were lighter than control group （ 18.5 ± 1.1 ）g from 2nd to 8th week（P〈0.01 ）. After chronic administration of escitalopram, the body weight of cms ＋ Cesc group （ 18.5± 0.6 ） g were increased relative to other stressed groups （P〈 0.01 ）. （2）In open-field test for 15rain, the total distance and central distance of stressed groups were further, and numbers of rear were more than control group （P 〈 0.05 ） from 2nd to 4th week, but then these indexes were decreased from 6th to 8th week （P 〈 0.05 ）. After chronic administration, the total distance （57.4 ±11.0） m, central distance（ 12.0 ± 3.0） m and numbers of rear（74.2 ± 6.1 ） of cms ＋ Cesc group increased （P 〈 0.05 ）. （3） In sucrose preference test, the preference percentage of stressed groups was less than control group （P 〈 0.05 ） from 6th to 8th week at 8th week, the preference percentage of cms ＋ Cesc group （ （79.0 ± 2.7 ） % ） was more than other stressed groups （P 〈 0.05 ）. （4） In forced swimming test, compared with control group, immobility time of stressed groups became longer from 4th to 8th week.. After chronic administration,immobility time of cms ＋ Cesc group（ （124.7 ±8.00）s） was shorter than other stressed groups. （5）After acute administration, the behavior of cms ＋ Aesc group was not different relative to pure stressed group（P〉 0.05）. Conclusion Chronic administration of escitalopram show up efficacy on depression behavior of CUMS mice, but acute administration is ineffective.