依达拉奉、神经节苷酯对帕金森病模型大鼠的神经保护作用

The neuroprotection of edaravone and GM1 on the rat model of parkinson disease

目的探讨依达拉奉（edaravone，Ed）、神经节苷酯（GW1）对帕金森病（PD）大鼠模型的神经保护作用。方法采用立体定向--N黑质致密部（SNC）、中脑腹侧被盖部（VTA）两点注射6-OHDA的方法，建立单侧PD大鼠动物模型。将老年大鼠分为正常组、生理盐水组（NS）、帕金森病模型组（PD）、PD＋GMI组、PD＋Ed组、PD＋GM1＋Ed组。14d后观察大鼠阿朴吗啡（APO）诱导的行为学改变及黑质多巴胺能神经元凋亡的情况。结果正常组、Ns组APO未诱导出大鼠旋行为，SNC未见细胞凋亡。PD各组APO诱导的旋转实验〉7r／min，旋转次数（次）PD＋GM1＋Ed组（8．0±0．3）〈PD＋Ed组（12．0-4-0．6）〈PD＋GM1组（17．0±1．0）〈PD组（23．0±1．3）（P〈0．01）；6-OHDA可诱导细胞凋亡，毁损侧SNC细胞凋亡数（个）PD＋GM1＋Ed组（27．63±2．38）〈PD＋Ed组（38．42±3．54）〈PD＋GM1组（49．36±3．12）〈PD组（62．61±4．03）（P〈0．01）。结论6-OHDA可导致大鼠行为改变，诱导SNC神经细胞凋亡，GM1、依达拉奉降低了这种损害，依达拉奉作用强于GM1，联合治疗作用最佳。

Objective To investigate the protect effects of Edaravone（Ed） and GM1 on the rat model of parkinson disease（PD）. Methods To establish the unilateral PD rat model ,6-OHDA was injected at two points of right substantial nigra pars compacta （SNC）, ventral tegmental area （VTA）, then the old rats were randomly divided into normal,NS,PD,PD ＋ GM1 ,PD ＋ Ed,PD ＋ GM1 ＋ Ed six groups. 14d later, a rotational test induced by apomorphiue was performed to determine the successful ratio. Cell apoptosis in SNC of rats were examined by TUNEL methods. Results Normal and NS groups unappeared rotate action by APO,and have no cell apoptosis in SNC. The other groups all appear rotate action（ 〉 7 r/min）by APO,rotate action were in following gradation：PD ＋ GM 1 ＋ Ed group（ 8.0 ± 0.3 ） 〈 PD ＋ Ed group （ 12.0 ± 0.6 ） 〈 PD ＋ GM1 group （ 17.0 ± 1.0） 〈 PD group （ 23.0± 1.3） （P〈0.01） ;and cell apoptosis in SNC were in following gradation：PD ＋ GM1 ＋ Ed group（27.63 ±2.38） 〈 PD ＋ Ed group（ 38.42 ±3.54） 〈 PD ＋ GM1 group（49.36 ± 3.12） 〈 PD group（62.61 ±4.03） （P〈0.01）. Conclusion 6-OHDA could induce change of action of rat and cell apoptosis in SNC. GM1, Ed reduce signifi- cantly the effect induced by 6-OHDA. GM1 combining with Ed have the best effects.