不同时程心理应激对支气管哮喘大鼠免疫功能的影响

Effects of Different Procedures of Psychological Stress on Immune Function of Rats with Bronchial Asthma

目的:探讨幼年和成年期心理应激对成年支气管哮喘大鼠免疫功能的影响及其生理机制。方法:将32只Wistar幼年大鼠随机分为哮喘模型组(成年期制备哮喘模型)、成年应激哮喘组(成年时给予心理应激的哮喘大鼠)、幼年应激哮喘组(幼年时给予心理应激的哮喘大鼠)和正常对照组(n=8)。建立心理应激与哮喘大鼠模型,肺组织HE染色与白细胞亚群计数,放免法测定血清IL-4、皮质醇、肺泡灌洗液IgE及脑组织IL-1β含量。结果:束缚应激可加重哮喘动物气道炎症和多项免疫指标紊乱。幼年应激后幼年应激哮喘组血清皮质醇显著高于其他各组(P<0.01),哮喘造模结束后成年应激哮喘组大鼠中枢IL-1β和血清皮质醇含量升高,幼年应激哮喘组降低。结论:幼年与成年心理应激均加重了大鼠哮喘,但存在不同机制,HPA轴在这一过程中起了重要作用。

Objective：This study aims to explore the effects of childhood and adulthood psychological stress on adult asthma and the underlying mechanisms in rats.Methods：Thirty-two Wistar rat pups were randomly assigned to 4 groups of 8 each—the asthma group（establishing asthma model in adulthood）,the adulthood-stressed asthma group（establishing asthma and adulthood psychologially stressed model in adulthood）,the childhood -stressed asthma group（establishing early -life psychologially stressed model and asthma in adulthood） and the control group.Asthma and psychologically stressed rat models were established.Right lung tissues of rats were stained with hematoxylin and eosin（HE） and leukocyte differentiation were determined.Serum IL-4,cortisol,immunoglobulin E（IgE） in bronchoalveolar lavage fluid and IL -1β in brain were tested by radioimmunoassay.Results：Restraint stress induced airway inflammation and the disorder of several immune indicators.After early-life stress,serum cortisol level in the childhood-stressed asthma group was higher than other groups（P〈0.01）.After the asthma model was established,the adult stressed group demonstrated elevated serum cortisol level,while cortisol concentration in the childhood stress group was lowered.Conclusion：Exposure to stress in childhood and adulthood engaged different mechanisms in the exacerbation of asthma.The HPA axis plays an important role in this process.