聚氨酯软骨-骨双层复合材料的构建及性能研究

Study on the construct and properties of polyurethane cartilage and bone bilayered composite

关节软骨损伤是临床上的常见病,由于其组织再生能力差,可能导致骨性关节炎的发生,因此,研究开发骨-软骨移植替代材料非常重要。目的就是设计一体化软骨-骨双层复合材料,以解决软骨与骨的整合问题。该双层复合体上层软骨材料为聚氨酯,软骨下骨为羟基磷灰石/聚氨酯复合支架材料,两层结构中引用了同一种材料——聚氨酯,将双层结构有机黏合在一起,使黏合更牢固。下层多孔HA/PU复合支架材料的孔与孔之间相互贯通,孔隙率约为83%,孔径范围分布在200～600μm。体外细胞相容性实验表明,该一体化双层复合材料为细胞的黏附、增殖以及生存活力的维持提供了有利环境。上述结果表明该双层复合材料有望用于软骨组织工程修复。

Damage to articular cartilage is one of the most common diseases in clinic,and may lead to the development of osteoarthritis because this tissue has poor regenerative capacity.Therefore,exploring a suitable osteochondral substitute transplanting graft is of great importance.The aim of the present work was to make a bilayered construct by integrating a tissue-engineered cartilage into an underlying bony substrate in order to solve the integration problem of cartilage and subchondral bone.The cartilage material was polyurethane and the underlying bony substrate was hydroxyapatite/polyurethane composite scaffold,and the same material,that is polyurethane,used in both structures integrated two layers together and made the conglutination more firmly.The subchondral part of the bilayered construct had an interconnected porous structure with a porosity of 83% and pore size ranging from 200μm to 600μm.In vitro biocompatibility tests showed that the incorporated bilayered construct provided a favorable environment for initial cell adhesion,and maintained cell viability and cell proliferation.The results suggest that the bilayer material has the potential for further application in osteochondral tissue engineering.