热压协同处理对接种于鸡腿菇中的细菌芽孢灭活效果的影响

Inactivation Effects of Coprinus Comatus Inoculated with Bacterial Spores by High-Pressure Thermal Processing

以鸡腿菇为原料,以凝结芽孢杆菌芽孢和嗜热脂肪芽孢杆菌芽孢为对象菌,研究中温与超高静压协同处理条件下两种对象菌的灭活效果及其相应的动力学变化趋势。研究结果表明:在相同条件下,鸡腿菇中凝结芽孢杆菌芽孢的致死效果优于嗜热脂肪芽孢杆菌;接种于鸡腿菇中的两种对象菌芽孢致死效果均低于磷酸缓冲液中的效果,说明鸡腿菇对这两种细菌芽孢有一定的保护作用。利用线性模型、Weibull模型和Log-logistic模型,对鸡腿菇中两种对象菌芽孢的灭活效果进行拟合,以均方差和回归系数为评价指标,分析了3种模型的拟合效果。结果表明:在所研究的条件下,Log-logistic模型的拟合效果最好,Weibull模型次之,线性模型最差。因此可以通过Log-logistic模型来预测热压协同处理下细菌芽孢的灭活效果。

The combination effect of high hydrostatic pressure and moderate heat on the inactivation effects of coprinus comatus inoculated with bacterial spores was investigated. The results showed that two bacterial spores （Bacillus coagulans and Bacillus stearothermophilus） had a much higher resistance in coprinus coma- tus than in phosphate buffer solution. Moreover,Bacillus stearotherrnophilus spore had a much higher resist- ance than Bacillus coagulans spore at the same pressure and temperature. Survival data were modeled using the linear,Weibull and Log-logistic models to obtain relevant kinetic parameters. The mean square error and regression coefficient suggested that the Log-logistic model produced best fits to all survival curves,following by Weibull model, and the linear model was not adequate for describing these curves. The Log-logistic model could be used to forecast the inactivation curves of bacterial spores by the combination of high hydrostatic pressure and moderate heat.