不同来源丹参种质遗传多样性的SRAP标记分析

SRAP Analysis of genetic diversity about germplasm in Salvia miltiorrhiza from different sources

目的解决丹参育种工作中的丹参种质资源的鉴别与分类。方法利用SRAP分子标记技术对48个不同来源的丹参种质进行了遗传多态性的分析,并对其进行了类群的划分。结果利用筛选到的15对SRAP引物组合从材料中检测到了120个等位位点。利用不加权成对群算术平均法(UPGMA)对丹参种质进行聚类分析可以将其分为两大类群,每一类群包含3个亚群。结论不同丹参种群间遗传距离与空间距离之间的关系较为复杂,不同地区间丹参种质的遗传分化不均衡。随着丹参种质驯化程度的增加,种质间的遗传距离有所增加。

Objective To solve the key problem on classification and identification of Salvia miltiorrhiza, which was urgently needed to be solved in breeding of S. miltiorrhiza. Methods Sequence-related amplified polymorphism （SRAP） was carried out to analyze the genetic variation of 48 germplasm in S. miltiorrhiza from different sources. Results The data showd that 120 alleles had been found using 15 pairs of primer which had been selected from 100 pairs. Unweighted pair-group method with arithmetical averages （UPGMA） cluster analysis was used to classify the germplasm of S. miltiorrhiza. Two groups could be divided and three subgroups were contained in every group. Conclusion A complex relationship exists between the genetic distance and space distance of differernt S. miltiorrhiza. The genetic diversity in different geographical populations of S. miltiorrhiza in China is rich. The results show that the genetic distance of different germplasm will increase with the stage of domestication.