辐射损伤小鼠胸腺组织的FTIR光谱变化与辐射剂量的关系

Study on Estimation of Radiation Dose Using FTIR in Mice Thymus

应用傅里叶变换红外(FTIR)光谱分析辐射损伤小鼠胸腺组织随辐射剂量增加的生化变化过程,为临床评估患者辐射剂量提供新的研究思路。结果表明,随着辐射剂量的增加,辐射损伤小鼠胸腺组织FTIR光谱的主要吸收峰位变化表现在照射2,3,5Gy时,在1550,1460,1400及1640cm-1处峰位发生移位(P<0.05),峰高差异表现在:(1)与核酸有关的谱带1085和1236cm-1的峰强比呈下降趋势,(2)与蛋白质有关的谱带1640与1550cm-1的峰强比随剂量增加明显下降,(3)与脂类有关的谱带2958,2925,1460及1400cm-1未发生明显的变化。研究结果表明傅里叶变换红外光谱有望成为临床估算受照剂量的新方法。

Fourier transform infrared spectroscopy （FTIR） was applied to study the biochemical changes in the radiation damaged mouse thymus which increased with radiation dose and provided a new method for the estimation of the radiation dose of radiation damaged patients. The results demonstrated that with the dose increasing, the peak positions like 1 550, 1 400, 1 400 and 1 640 cm^-1 at the dose of 2, 3 and 5 Gy showed some difference, and there was obvious variance in the intensity： （1） The intensity ratio of 1 085 to 1 236 cm^-1 related to nucleic acid tended to decrease. （2） The intensity ratio of 1 640/1 550 decreased. （3） The intensity at 2 958, 2 925, 1 460 and 1 400 cm^-1 showed no significant difference. The results suggest that it may be possible for FTIR to become an effective method to estimate the radiation dose in clinic.