近红外光谱技术(NIRS)在检测牧草霉菌毒素中的应用

Applications of Near Infrared Reflectance Spectroscopy Technique to Determination of Forage Mycotoxins

具有快速、高效、无损、在线等优势的近红外光谱技术(nearinfrared reflectance spectroscopy technique,NIRS)已经在农业、食品、化工、医学等领域获得广泛应用。牧草在加工贮藏过程中,受多种真菌的侵染,引起霉菌毒素在牧草中积累。霉菌毒素会通过动植物产品进入人畜的食物链,引起人畜中毒。牧草中霉菌毒素的常规检测不但需要粉碎、浸提、层析等繁琐的样品前处理过程,还需要酶联免疫吸附法、高效液相色谱法、溥层色谱法等后续检测过程。通过发展高精度、低检出限的光谱仪器,建立相应霉菌毒素检测的软件技术与校正模型,能够在牧草利用中达到快速准确检测霉菌毒素的含量和性质,从而为牧草的合理加工与利用提供依据,促进NIRS技术在健康畜产品生产领域的应用。

The near infrared reflectance spectroscopy technique （NIRS） has been explored at many fields such as agriculture,food,chemical,medicine,and so on,due to its rapid,effective,non-destructive,and on-line characteristics. Fungi invasion in forage materials during processing and storage would generate mycotoxins,which were harmful for people and animal through food chains. The determination of mycotoxins included the overelaborated pretreatments such as milling,extracting,chromatography and subsequent process such as enzyme linked immunosorbent assay,high performance liquid chromatography,and thin layer chromatography. The authors hope that high precision and low detection limit spectrum instrument,and software technology and calibration model of mycotoxins determination,will fast measure accurately the quality and quantity of mycotoxins,which will provide basis for reasonable process and utilization of forage and promote the application of NIRS in the safety livestock product.