Th/Tr比值评估结直肠癌患者抗肿瘤免疫状态的临床价值

THE VALUE OF Th/Tr LYMPHOCYTE RATIO IN EVALUATING THE ANTICANCER IMMUNE STATUS IN THE PATIENTS WITH COLORECTAL CARCINOMA

目的检测结直肠癌患者外周血中T-heper和T-reg细胞的比值(Th/Tr),探讨其评估患者抗肿瘤免疫状态的意义。方法应用流式细胞技术检测60例结直肠癌患者和30例健康者外周血Th/Tr比值,分析结直肠癌患者与健康者外周血 Th/Tr是否存在差异,不同临床分期、不同病理分级结直肠癌患者外周血Th/Tr值是否存在差异。结果结直肠癌患者外周血Th/Tr值为5.28±2.43,低于健康对照组的11.24±1.84(P<0.01);早期患者外周血Th/Tr为6.64±2.39,高于进展期患者的4.71±2.16(P<0.01),Th/Tr比值与病理分级无关。结论结直肠癌患者外周血中Th/Tr降低,提示结直肠癌患者的免疫受损,其水平变化可作为评估结直肠癌患者预后的指标之一。

Objective To investigate the ratio of T-helper/T-reg lymphocyte（Th/Tr） and its role in evaluating the antieancer immune status in the patients with colorectal carcinoma. Methods Peripheral blood was collected from 60 patients with colorectal carcinoma and 30 healthy donors. The flow eytometry was used to detect the percentages of Th and Tr cells. Results The ratio of Th/Tr in peripheral blood was significantly lower in the patients with colorectal cancer than in the healthy donors （5.28±2.43 vs 11.24±1.84, P 〈0.01） and was significantly higher in the patients with early stage of cancer than in those with advanced cancer （6.64±2.39 vs 4.71 ±2.16, P〈 0.01）, whereas it was not correlated with pathological differentiation. Conclusion The declination of Th/Tr ratio suggests the impairment of immune activity in the patients with eoloreetal cancer and seems to be a useful immune parameter to evaluate the prognosis of colorectal cancer.