摘要
针对甲型流感病毒H1N1基因,从RNAi的角度出发,采用多特征融合的方法,进行siRNA预测。对2009年的46株病毒序列的PA片段进行分析,从经过序列分析所获得的众多靶序列中,采用结构分析手段对靶序列进行筛选,获得较易干扰的靶序列及设计出相应的siRNA。2009年爆发的H1N1病毒,序列保守性高,靶序列一致性高,结构保守性高。该方法可以有效选择可能的靶序列,并在此基础上进一步筛选,以获得少量较易干扰的靶序列,该方法为复杂序列siRNA的设计提供了新思路,对siRNA的优化设计有指导意义,有助于利用RNAi进行H1N1治疗的后续研究。
Aiming at H1N1 gene and starting from the point of RNAi,siRNA prediction is conducted by means of multi-characters analyses,such as sequence and structure.First 46 viral sequence＇s PA fragments in 2009 are analyzed.Then the siRNA with strong ability of interference are selected on the basis of the secondary structure of target sequence.Our research reveals that the outbreaking H1N1 virus in 2009 is characterized by high sequence conservativeness,uniform target sequence,and high conservative structure.This method can be employed to choose the possible target sequence and obtain less but more valuable target sequence by further sifting.It provides a new idea for the design of complex sequence siRNA,and it is of instructional significance for optimal design of siRNA.The research is helpful to the study of H1N1 treatment by RNAi.
出处
《吉林大学学报（工学版）》
EI
CAS
CSCD
北大核心
2010年第3期776-781,共6页
Journal of Jilin University:Engineering and Technology Edition
基金
国家自然科学基金项目(60673099
60873146
60971089)
关键词
生物信息学
RNAI
SIRNA
二级结构
bioinformatics
RNA interfering
small interfering RNA
secondary structure
