产木糖醇菌株的筛选及发酵条件优化

Screening of xylitol-producing strain and optimization of its fermentation conditions

从玉米芯中筛选到1株高效转化D-木糖为木糖醇的酵母菌株e。经形态学鉴定及26S rDNA D1/D2区序列分析,将菌株e鉴定为Trichosporon coremiiforme(丝孢酵母)。采用单因素及正交试验优化设计研究了发酵法生产木糖醇的工艺,结果表明:酵母菌株e发酵法生产木糖醇的工艺参数为:碳源质量浓度为40g/l,氮源质量浓度为6g/l,起始pH为7,接种量为6%。

A yeast,which can perform biotransformation from xylose to xylitol,was isolated from corncob and was identified as trichosporon coremiiforme by morphological identification and 26S rDNA D1/D2 domain sequence analysis.Besides,the optimal fermentation conditions were determined through single factor test and orthogonal experiment.The optimal conditions are as follows,carbon source concentration：40g/l,nitrogen source concentration：6g/l,initial pH：7.0 and inoculum size：6%.