原发性胆汁性肝硬化患者外周血TNFSF9的表达及其疾病关联性

Expression of TNFSF9 in the Peripheral Blood Mononuclear Cells of Primary Biliary Cirrhosis Patients and Its Clinical Significance

目的研究原发性胆汁性肝硬化患者外周血单个核细胞TNFSF9的表达及其临床意义。方法以40例健康人群作为对照，采用定量逆转录PCR检测40例原发性胆汁性肝硬化患者外周血单个核细胞TNFSF9的表达水平，血清中sTNFSF9的水平，并进一步分析其与Mayo评分的相关性。结果原发性胆汁性肝硬化患者外周血TNFSF9的表达在蛋白水平（1107．00±436．12）显著高于健康对照组（122．70±62．81）；TNFSF9 mRNA水平（4．96±0．39）也较健康对照组（1．04±0．22）高，且均与Mayo评分呈负相关（rs=0．327，P=0．041；rs=-0．400，P=0．013）。结论TNFSF9可能参与了原发性胆汁性肝硬化的发病机制，是潜在的治疗靶点之一。

Objective To investigate the expression of TNFSF9 in the peripheral blood mononuclear cells （PBMC） of primary biliary cirrhosis（PBC） patients and its clinical significance. Methods 40 patients and matched healthy controls were enrolled retrospectively and the TNFSF9 concentration in their serum and the expression in PBMC were detected by realtime RT-PCR respectively. In addition,their relationships with Mayo score were analyzed. Results TNFSF9 expression was higher at both protein （1 107.00± 436.12） and mRNA（4.96 ± 0.39） level in PBC patients when compared with healthy controls （122.70±62.81 ; 1.04 ±0.22）. Moreover, TNFSF9 mRNA and protein level were negatively correlated with Mayo risk score （rs = 0. 327,P=0. 041;rs=-0. 400,P=0. 013）. Conclusion TNFSF9 may involve in the pathogenesis of PBC,and maybe a potential therapy target.