大肠癌明胶酶和TGF-β1及其受体的表达

Expression of Gelatinases and their Relationship with TGF-β1 and TGF-β1RⅠ in Colon Cancer

目的研究明胶酶(MMP-2,9)与转化生长因子(TGF-β1)及其Ⅰ型受体(TGF-β1RⅠ)在大肠癌组织中的蛋白表达及其相互关系。方法应用免疫组织化学S-P法检测89例大肠癌组织中MMP-2、MMP-9与TGF-β1、TGF-β1RⅠ蛋白的表达。结果大肠癌MMP-2、MMP-9、TGF-β1和TGF-β1RⅠ阳性率分别为67.4%、65.2%、85.4%和71.9%;MMP-2表达与肿瘤大小和淋巴结转移均呈正相关(均P<0.01),MMP-9表达与淋巴结转移呈正相关(P<0.01),TGF-β1RⅠ的表达与淋巴结转移呈负相关(P<0.01);TGF-β1表达状况与MMP-2和MMP-9的表达均呈显著正相关(r=0.256和0.365,P<0.05和0.001),TGF-β1RⅠ和MMP-9的表达呈显著正相关(r=0.225,P<0.05)。结论MMP-2,9的表达情况与大肠癌侵袭转移有密切关系。MMP-2,9的表达与TGF-β1、TGF-β1RⅠ关系密切,可能在一定程度上受到TGF-β1及其受体的调控。

Objective To investigate the protein expression of MMP-2,MMP-9,TGF-β1 and TGF-β1RⅠ and its clincopathological features and the relationship between them in colon cancer.Methods The protein expression of MMP-2,MMP-9,TGF-β1 and TGF-β1RⅠ were examined on tissue chips which containing 89 cases of colon carcinoma by S-P immunohistochemical method.Results The positive rates of MMP-2,MMP-9,TGF-β1 and TGF-β1RⅠ protein were 67.4%,65.2%,85.4% and 71.9%,respectively.The expression of MMP-2 was positively correlated to lymph node metastasis and tumor size（both P〈0.01）.The expression levels of MMP-9 was positively correlated to lymph node metastasis（P〈0.01）.The expression levels of TGF-β1RⅠ was negatively correlated to lymph node metastasis（P〈0.01）.Both the expression of MMP-2 and MMP-9 was positively correlated to that of TGF-β1（r=0.256 and 0.365,P〈0.05 and P〈0.01,respectively）,and MMP-9 was also positively correlated to the expression of TGF-β1RⅠ（r=0.225,P〈0.05）.Conclusion The expression of MMP-2 and MMP-9 might be close correlated to the invasion and metastasis in colon cancer.The expression of MMP-2 and MMP-9 had a close relationship with TGF-β1 and TGF-β1RⅠ.The levels of MMP-2 and MMP-9 might be partly regulated by TGF-β1 and TGF-β1RⅠ.