姜黄素对过氧化氢诱导的大鼠心肌细胞MCP-1表达的影响

Effects of curcumine on expression of MCP-1 induced by H_2O_2 in myocardial cell

目的:观察姜黄素(curcumine)对过氧化氢诱导的大鼠心肌细胞MCP-1表达的影响,探讨姜黄素在心肌细胞氧化应激中作用的可能机制。方法:以H2O2处理心肌细胞,构建心肌氧化损伤模型。并用姜黄素及H2O2共孵育心肌细胞,采用免疫印迹(Western blot)检测干预后不同时间MCP-1蛋白表达。结果:在干预后4、8、12、24、48h,H2O2组心肌细胞表达的MCP-1明显高于空白对照组(P<0.05),加入姜黄素共孵育后,各个时间点MCP-1的表达都明显下降(P<0.05)。结论:H2O2能诱导心肌细胞MCP-1蛋白表达,而姜黄素对H2O2诱导的心肌细胞MCP-1表达具有抑制作用,可能是其抗氧化应激的机制之一。

Objective：To investigate the effects of curcumine on expression of monocyte chemoattractant protein-1（MCP-1） induced by H2O2 in myocardial cell. To explore the possible mechanism of antioxygenation of curcumine in oxidative stress. Methods：In order to gain the oxidative injure cell model,cells myocardial with H2O2. Myocardial cells were incubated with curcumin and H2O2,then the expression of MCP-1 at different time points during the oxidative injure was detected by western blotting. Results：Western blotting showed that MCP-1 expressed in all groups. Higher level of MCP-1 expression was observed in H2O2 stimulated group compared with that in control group（P 〈 0.05）. After treated with curcumin,the expression of MCP-l in H2O2＋ curcumine group was reduced（P 〈 0.05）. Conclusion：The expressions of MCP-1 induced by H2O2 can be inhibited by curcumine,and it may be one of the mechanisms of antioxygenation effect of curcumine.