黄芪丹参对干燥综合征模型大鼠颌下腺的干预研究

Effects of Astragalus Danshen on rat submandibular gland in Sjogren's syndrome model:an intervention study

目的:观察中药黄芪合并丹参组成的小复方对干燥综合征模型大鼠颌下腺的干预。方法:提取Wistar同种大鼠颌下腺组织后匀浆与完全弗氏佐剂(CFA)混匀乳化,注射大鼠两后脚掌与两侧腹股沟皮下,然后在背部皮下注射百白破联合疫苗加强免疫,产生近似于人类干燥综合征唾液腺的病理改变及临床表现。应用中药颗粒黄芪合并丹参干预进行疗效观察。结果:模型大鼠免疫制备7W,唾液流量减少(P<0.05),体重明显减轻(P<0.05),颌下腺指数增加(P<0.05)。黄芪合并丹参对模型鼠部分观察指标有改善,造模中药复方低、高剂量组能明显增加唾液流量(P<0.05),增加体重(P<0.01),但是饮水量与造模后生理盐水对照组没有统计学意义(P>0.05)。结论:黄芪合并丹参小复方对复制干燥综合征模型鼠有增加体重、增加唾液分泌量的作用。

Objective：To observe the interventive effects of Astragalus Danshen compound on rat submandibular gland in a small model of Sjogren’s syndrome. Methods：Submandibular gland tissue was extracted from Wistar rat,and emulsificated with complete Freund’s adjuvant （CFA）after blending,then subcutaneously injected into the rats on the hind feet and both sides of the groin. After that,subcutaneous injection of DTP vaccine was performed on the back to enhance immunity,thus producing pathological changes and clinical manifestations of the salivary gland similar to human Sjogren’s syndrome. Traditional Chinese medicine Astragalus particles combined with Danshen were applied. Results：The rat model was treated for 7 weeks. Salivary flow was reduce（P 〈 0.05）,significant weight loss was found（P 〈 0.05）,and submandibular gland index increased（P 〈 0.05）. Partial parameters were imroved on rat model treated with Astragalus Danshen. The Chinese herbal compound significantly increased salivary flow（P 〈 0.05）,both in the low and high dose groups（P 〈 0.01）. However,there was no significance in water drinking between the rat model and the saline control group（P 〉 0.05）. Conclusion：Astragalus Danshen can increase body weight and saliva secretion in the rat model mimicking Sjogren’s syndrome.