CTLL-2/MTT法快速测定IL-2基因转移效率的研究

Transduction effect of interleukin2 gene fast tested by CTLL2/MTT method

应用包装ＩＬ－２基因的逆转录病毒载体，将ＩＬ－２基因导入小鼠成纤维细胞ＮＩＨ－３Ｔ３和小鼠黑色素瘤细胞Ｂ１６，建立了ＮＩＨ－３Ｔ３／ＩＬ－２－２，ＮＩＨ－３Ｔ３／ＩＬ－２－７和Ｂ１６／ＩＬ－２－４等３个转基因细胞株，应用ＣＴＬＬ－２／ＭＴＴ法快速测定各转基因细胞株分泌上清ＩＬ－２的浓度．转染效率最高的ＮＩＨ－３Ｔ３／ＩＬ－２－７其ＩＬ－２分泌量高达１４２２ｕ／（１０６ｃ·２４ｈ），表明这一基因转移系统能有效地将外源基因导入靶细胞．ＣＴＬＬ－２／ＭＴＴ法能快速、简便、有效地检测ＩＬ－２的表达．

Interleukin2(IL2) gene is transduced into melanoma cell and fibroblast using retroviral vector packaging IL2 gene. Three transduced cell lines which could express IL2 gene have been structured. CTLL2/MTT method is established to test IL2 expression level of transduced cell, the highest cecretory amount of which reaches 1 422 u/(106 c·24 h). The study shows that the retroviral mediated gene transduction system could introduce foreign gene into target cell effectively and conveniently as well as that CTLL2/MTT method is easy to operate and is also much safer and cheaper. This method is worth spreading.