支气管哮喘患儿外周血淋巴细胞CD19^+CD23^+和CD4^+CD25^+的表达及意义

Expression and significance of CD19^+CD23^+ and CD4^+CD25^+ in peripheral blood lymphocytes in children with bronchial asthma

目的探讨支气管哮喘患儿外周血淋巴细胞CD19+CD23+和CD4+CD25+的表达及意义。方法采用流式细胞分析技术及免疫化学发光法分别检测34例哮喘发作期(哮喘发作组)和32例哮喘缓解期(哮喘缓解组)外周血CD19+CD23+表达率、CD4+CD25+表达率和血清总IgE水平,并与25例体检儿童(对照组)进行比较。结果哮喘发作组外周血CD19+CD23+表达率为(19.31±9.08)%分别高于哮喘缓解组(8.63±3.54)%和对照组;哮喘发作组CD4+CD25+表达率为(2.79±1.69)%和哮喘缓解组为(3.36±1.17)%均低于对照组;哮喘发作组IgE水平为(177.01±90.38)IU/ml和哮喘缓解组为(160.76±77.54)IU/ml均高于对照组,三组的差异均有统计学意义(F分别=36.62、24.67、24.35,P均<0.05)。CD19+CD23+表达率与IgE呈正相关,CD4+CD25+表达率和IgE呈明显负相关,CD19+CD23+表达率和CD4+CD25+表达率亦呈明显负相关,差异均有统计学意义(r分别=0.22、-0.41、-0.34,P均<0.05)。结论哮喘急性发作时外周血CD19+CD23+和CD4+CD25+改变明显。

Objective To investigate the expression and significance of CD19＋CD23＋ and CD4＋CD25＋ in peripheral blood lymphocytes in children with bronchial asthma. Methods A total of 34 children with asthma, 32 with remitting asthma, and 25 healthy children were subject to detect the expressions of CD19＋CD23＋and CD4＋CD25＋in peripheral blood lymphocytes by flow cytometer and the levels of serum IgE by chemiluminescence immunoassay. Results The expressions of CD19＋CD23＋In peripheral blood lymphocytes of children with asthma [（19.31±9.08）%] were significantly higher than those of children with remitting asthma [（8.63 ±3.54）% ] and healthy ones.The expressions of CD4＋CD25＋ in peripheral blood lymphocytes of children with asthma [（2.79±1.69）%] and remitting asthma [（3.36± 1.17）%] were lower than those of the healthy ones. The serum IgE of the healthy children were lower than those of the children with asthma [（177.01±90.38）IU/ml] and remitting asthma [（160.76±77.54）IU/ml]. There were statistically significant differences （F=36.62, 24.67, 24.35, P〈0.05）. There was a positive correlation between the expressions of CD19＋CD23＋in peripheral blood lymphocytes and serum IgE and a negative correlation between the expressions of CD4＋CD25＋ and serum IgE. There was also a significantly negative correlation between the expressions of CD19＋CD23＋ and CD4＋CD25＋ in peripheral blood lymphocytes. The differences were statistically significant （r= 0.22, -0.41, -0.34,P〈0.05）. Conclusions The change of the expressions of CD19＋CD23＋ and CD4＋CD25＋in peripheral blood lymphocytes in children with bronchial asthma is obvious.