蜘蛛染色体制片技术探索

SMEARING TECHNOLOGY FOR SPIDER CHROMOSOME

本文以多种蜘蛛不同的组织器官为实验材料，通过设置不同低渗处理时间和采用多种固定的方法，旨在找到蜘蛛染色体制片技术的最优化方案．结果表明：选取材料以蜘蛛处于发育中的生殖腺和幼蛛的附肢细胞为最好．在无水浴条件下，低渗时间对取血细胞为40分钟，对幼蛛取45分钟为最佳．用新鲜固定液进行固定，对第一次固定加以改进，采用高比例甲醇固定剂处理，有利于染色体伸展和染色体分散，得到理想的分裂相．

In order to find the best smearing technology for spider chromosome,experiments have made by using different tissues and organs of diverse spiders as experimental materials,and designing different low-osmosis work-time and diverse fixed methods. Results indicate that the growing spider's reproduction gonad and young spider's appendage cell are thebest experimental materials; without water-bath, the low-osmosis work-time is 40 minutesfor blood cell (but 45 minutes for young spiders),and when high concentrated methanol isused as the first fixing-solution, it is of advantage for extension and dispersion of chromosome,and it is easy to gain the satisfactory divided-phase.