α-促黑素细胞激素对IL-1β刺激离体兔下丘脑释放PGE_2的抑制作用

α-Melanocyte Stimulating Hormone Inhibits PGE 2 Release from Rabbit Hypothalamus Induced by rhIl-1 β in Vitro

采用体外组织培养法观察α－促黑素细胞激素（α－ＭＳＨ）对白细胞介素－１β（ＩＬ－１β）刺激家兔下丘脑组织释放前列腺素Ｅ２（ＰＧＥ２）的影响。结果显示，ＩＬ－１β与下丘脑组织培养后４０ｍｉｎ，培养上清液中ＰＧＥ２含量高于未加ＩＬ－１β的对照组（Ｐ＜００１）；而预先（２０ｍｉｎ）加入α－ＭＳＨ，再加入同量ＩＬ－１β培养４０ｍｉｎ，则培养上清液的ＰＧＥ２含量接近对照组水平（Ｐ＞００５）。α－ＭＳＨ抑制下丘脑ＰＧＥ２的产生，可能是其解热的重要机制之一。

A tissue culture technique was used to evaluate the effect of α-MSH on IL-1 β-induced PGE 2 release from hypothalamus of rabbit in vitro. The result showed that the PGE 2 contents of culture supernatant increased significantly after hypothalamic blocks were incubated for 40 min in the presence of rhIL-1 β( P <0 01),and the effect could been prevented by α-MSH added to the incubation solution 20 min in advance. It was considered that the effect of α-MSH inhibiting PGE 2 production by hypothalamus may be an important mechanism in α-MSH-induced antipyresis.