摘要
Objective To investigate whether estrogen modulates learning and memory and long-term potentiation （LTP） in the hippocampus of rats with Alzheimer＇s disease （AD）. Methods The rats were divided into ovariectomy （OVX） and estrogen replacement therapy （ERT） groups. Rats in the ERT group received OVX, followed by ERT, while rats in the OVX group received only OVX. The rat model of AD was established by injection of 1 μL （10 μg/μL） amyloid-beta peptide 1-40（Aβ1-40） into the hippocampus. The learning and memory ability and LTP were determined by Morris water maze and electrophysiological method, respectively. Results The escape latency in Morris water maze significantly decreased in ERT group compared with that in OVX group （P 〈 0.05）. Besides, rats in ERT group exhibited a significant enhancement of the magnitude of LTP at 30 min after high-frequency stimulation （HFS）, compared with that in OVX group （P 〈 0.01）. Conclusion ERT can attenuate the cognitive deficits in the rat model of AD, and estrogen can regulate LTP and synaptic remodeling in AD rats.
目的探讨雌二醇对阿尔茨海默病(Alzheimer's disease,AD)大鼠的学习记忆能力及长时程增强(long-term potentiation,LTP)的影响。方法大鼠随机分成卵巢切除(ovariectomy,OVX)组和雌激素替代治疗(estrogen replace treatment,ERT)组。两组大鼠均接受卵巢切除手术,ERT组大鼠随后接受雌激素替代治疗。手术2周后,两组大鼠海马内立体定位单侧注射1μLAβ1-40(10μg/μL),建立AD模型。运用水迷宫实验检测大鼠的学习和记忆能力,并进行LTP记录和比较。结果OVX组AD大鼠的水迷宫逃避潜伏期相较于ERT组明显延长(P<0.05)。此外,高频刺激(highfrequence stimulation,HFS)30min后与HFS刺激前相比,ERT组LTP增加的幅值明显高于OVX组(P<0.05)。结论雌激素替代治疗能改善AD大鼠的认知功能,调整LTP及突触可塑性。
基金
supported by the National Natural Science Foundation of China (No. 30700938,30872656, 30700861, 30800451)
the Natural Science Foundation of Liaoning Province, China (No. 20082078)
