羟氯喹治疗口腔扁平苔藓前后的唾液双向电泳研究

saliva two-dimensional gel electrophoresis analysis about hydroxychloroquine action on oral lichen planus

目的:比较口腔扁平苔藓(OLP)经羟氯喹治疗后,唾液中差异表达的蛋白质,探讨羟氯喹治疗OLP的可能作用机制。方法:采用固相pH梯度(immobilized pH gradient,IPG)双向凝胶电泳(two-dimensional gel electrophoresis,2-DE)分离OLP经羟氯喹治疗三个月后唾液中的差异蛋白质,银染显色,Imagemaster图像分析软件分析差异蛋白质点。结果:①羟氯喹治疗OLP前和治疗后的唾液2-DE图谱的平均蛋白质点数分别为1894±183个和1920±341个,凝胶图谱分辨率、重复性较好;②通过Imagemaster图像分析比较治疗前后的唾液2-DE图谱,差异表达的蛋白质点数为21个,其中6个点在治疗3个月后高表达,15个点在治疗后低表达。结论:本研究建立了分辨率高且重复性较好的OLP治疗前后的唾液双向凝胶电泳图谱,发现存在差异表达的蛋白质,为进一步研究羟氯喹治疗OLP的作用机制奠定了基础。

Objective：To compare the difference of the serum protein about the patient of oral lichen planus who received hydroxychloroquine therapy and to explore its possible mechanism.Method：The total serum proteins of oral lichen planus who received hydroxychloroquine therapy were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis（2-DE）.After AgNO3 staining,the differential expression proteins were ana1yzed by using ImageMaster image ana1ysis software.Result：（1）The well-resolved,reproducible 2-DE patterns of the serum of oral lichen planus who received hydroxychloroquine therapy were obtained.The results showed that average spots before and after the medicine treatment were 1894±183 and 1920±341 respectively;（2）The 21 differential protein spots were identified by ImageMaster image analysis software.There were 6 protein spots were higher expression、15 protein spots were lower expression after the hydroxychloroquine treatment.Conclusion：In this study,the well-resolved reproducible 2-DE patterns of the serum of oral lichen planus before and after the treatment of hydroxychloroquine were established and some differential proteins were characterized,which will provide a basis for elucidating the mechanism of hydroxychloroquine action on oral lichen planus.