色素万寿菊叶片再生体系的建立及优化

The Establishment and Optimization of Leaves Regeneration System of Pigment Marigold

以色素万寿菊叶片为外植体,探讨了不同的植物生长调节剂组合、AgNO3、凝胶剂及不同的继代培养基对叶片器官发生的影响。结果表明:6-BA3.0mg·L-1+IAA3.0mg·L-1+蔗糖30.0g·L-1+植物凝胶6.0g·L-1的MS培养基适宜不定芽的分化,不定芽分化率达97.5%,将分化13d的叶片外植体转接到组织培养瓶中,30d后56.7%的不定芽可发育成高约5cm的植株,再生植株在MS培养基上生根率为100%,移栽成活率达100%。

An efficient plant regeneration protocol for Tagetes erecta L. was achieved via organogenesis from leaf explants in vitro. In this paper,the effects of different combinations of plant growth regulators,the addition of AgNO3,gel and the subculture medium on callus induction and buds regeneration were investigated. The research results indicated that the optimal media for buds regeneration is MS ＋ 6-BA 3.0 mg · L-1 ＋ IAA 3.0 mg · L-1 ＋ sucrose 30.0 g · L-1 ＋ phytagel 6.0 g · L-1 with the buds induction frequency of 97.5%. Put the 13-day-old explants with buds to the tissue culture flask,and 56.7% of them can grow up to 5 cm high after 30 days culture. The rooting of rootless shoots could be accomplished directly in the MS media with rooting frequency of 100%. All of the rooted shoots survived in greenhouse.