水迷宫实验中三种品系小鼠学习记忆能力的比较

Comparison of Learning and Memory Abilities among Three Species of Mice Tested in Morris Water Maze

目的探讨三种品系小鼠在Morris水迷宫实验中的学习记忆能力差异,为与改善学习记忆能力相关新药研究的实验动物选择提供参考。方法选用C57BL/6小鼠、昆明种小鼠和ICR小鼠,进行定位航行实验(5d)、空间搜索实验(1d)和工作记忆实验(4d),考察其学习记忆能力。结果定位航行实验从第3天起C57BL/6小鼠的潜伏期明显小于昆明种小鼠和ICR小鼠(P<0.01);空间搜索实验C57BL/6小鼠穿过原平台位置的次数、在原平台象限游程比率和时间比率均明显多于昆明种小鼠和ICR小鼠(P<0.05);动物每天工作记忆实验的潜伏期随测试次数增加而缩短,C57BL/6小鼠缩短的较昆明种小鼠和ICR小鼠稍多。结论C57BL/6小鼠的空间参考记忆能力与工作记忆能力均优于昆明种小鼠和ICR小鼠,且非空间因素对其学习记忆能力的评价干扰较小,可作为优先选择的水迷宫实验动物。

Objective To investigate the differences of learning and memory abilities among three species of mice tested in Morris water maze.Methods Place navigation,spatial probe and working memory tests were performed to determine the learning and memory abilities of C57BL /6,Kunming and ICR mice.Results C57BL /6 mice found the hidden platform faster than other two strains of mice in the place navigation test,showing a significant difference from the 3rd day（P 0.01）.In the spatial probe test,C57BL /6 mice spent more time in the target quadrant than other groups（P 0.001）.In working memory test,the escape latency of mice became shorter and shorter in four tests per day,and C57BL /6 mice saved more time to the platform than Kunming mice and ICR mice,but there was no significant difference between Kunming mice and ICR mice.Conclusions C57BL /6 mice perform better than Kunming mice and ICR mice in spatial reference and working memory abilities.It may be more suitable for Morris water maze test.