宫颈癌组织中Rap1GAP蛋白表达及其与HPV感染的相关性

Rap1GAP protein expression in cervical carcinoma and its relationship to HPV infection

[目的]探讨Rap1GAP蛋白在宫颈癌组织中的表达变化以及其与HPV感染之间的关系。[方法]免疫组化方法检测20例宫颈癌与20例正常宫颈组织HPV感染和Rap1GAP蛋白表达情况。[结果]HPV检出阳性率在正常宫颈组织和宫颈癌中分别为20%与75%,宫颈癌组明显高于正常宫颈组(P<0.05)。宫颈癌组的Rap1GAP蛋白表达阳性率为15%、弱阳性率为15%,二者均明显低于正常宫颈组(阳性率,55%和弱阳性率,45%)(P均<0.05)。宫颈癌组织中,HPV阳性组Rap1GAP蛋白缺失率为80%,明显高于HPV阴性组的缺失率40%(P<0.05),且二者之间呈正相关(r=0.553,P<0.05)。[结论]宫颈癌组织Rap1GAP蛋白表达下调或缺失可能与HPV感染有关。

[Objective]To reveal the change of Rap1GAP protein expression in cervical carcinoma and its relationship to HPV infection.[Methods]HPV and the expression of Rap1GAP protein in 20 samples of normal cervix and 20 samples of cervical carcinoma were detected by immunohistochemistry.[Results]The HPV infection rate was 75% in cervical carcinoma,higher than that of 20% in normal cervix（P〈0.05）.The positive and weak positive expression rates of Rap1GAP protein were 15% and 15% in cervical carcinoma,significantly lower than those of 55%（P〈0.05） and 45%（P〈0.05） in normal cervix tissues.In cervical carcinoma,deletion rate of Rap1GAP protein expression in HPV-positive samples was 80%,significantly higher than that of 40% in HPV-negative（P〈0.05）and there was a positive correlation between the deletion of Rap1GAP protein and HPV infection（r=0.553,P〈0.05）.[Conclusion]Expression of Rap1GAP protein is down-regulated or deleted in cervical cancer and it has a positive correlation with HPV infection,implying that there is a relationship among HPV infection,Rap1GAP protein expression deletion and cervical cancer.