三种不同方法检测鸡马立克氏病毒的效果比较

COMPARISON OF POLYMERASE CHAIN REACTION AMPLIFICATION (PCR), DOT HYBRIDIZATION AND VIRAL ISOLATION FOR DETECTING MAREK'S DISEASE VIRUS

研究比较了核酸斑6杂交技术、多聚酶链反应（PCR）扩增和病毒分离技术在检测MDV中的效果和相关性，试验结果表明：以PCR扩增I型MDV132bp重复序列或以此序列标记的Digoxigenin探针进行Dot－DNA分子杂交检测MDV，均具有较好的效果，灵敏度比常规病毒分离高近万倍，其特异性较强，并能快速鉴别MDVI型毒，使诊断时间缩短了许多。

Sensitvity of detecting March's disease virus (MDV) for the peripherial blood lymphocytes of chickens infected with woodlands No1 and CR/6 strains of MDV were compared among PCR amplification, dot-hybridization and viral isolation. The results showed that PCR amplification of 132 bp repeat sequence of MDV or dot-hybridization were more sensitive than viral isolation. The sensitivity of PCR and dot-hybridization were 103~104 times as high as viral isolation. Both of them demonstrated the high specificity and could be used to distinguish serotype 1 from other two serotypes of MDV. It is suggested that PCR and dot-hybridization were very useful in clinical diagnosis in the future.