钙激活氯通道和黏蛋白在变应性鼻炎中的表达与意义

Expression and clinical significance of calcium-activated chloride channel and mucins in nasal mucosa with allergic rhinitis

目的:探讨人钙激活氯通道1(hCLCA1)和黏蛋白MUC5AC在变应性鼻炎黏液高分泌中的表达水平及临床意义。方法:采用RT-PCR技术和免疫组织化学法,检测20例变应性鼻炎患者(变应性鼻炎组)和7例正常人(对照组)hCLCA1和MUC5AC在下鼻甲黏膜的表达水平。结果:变应性鼻炎组鼻黏膜hCLCA1mRNA表达阳性,而对照组未出现hCLCA1mRNA的表达,两者比较差异有统计学意义(P<0.01);变应性鼻炎组MUC5ACmRNA表达和MUC5AC蛋白表达均较对照组明显增多(均P<0.01);变应性鼻炎组鼻黏膜hCLCA1mRNA表达与MUC5ACmRNA和蛋白表达均呈直线正相关(r=0.752、0.694,均P<0.05)。结论:hCLCA1mRNA表达的上调可能在变应性鼻炎黏液高分泌中起着关键作用,抑制hCLCA1的表达将为变应性鼻炎的治疗提供新的策略。

Objective：To evaluate the role of human calcium-activated chloride channel 1（hCLCA1）and mucin MUC5AC in allergic rhinitis.Method：The expression of hCLCA1 and MUC5AC were determined by RT-PCR and immunohistochemical assay in nasal mucosa on 20 patients with allergic rhinitis and 7 normal persons.Result：The expression of hCLCA1mRNA in allergic rhinitis group was positive,whereas in normal control group was absent（P0.01）.The expression of MUC5AC mRNA and MUC5AC protein in the allergic rhinitis group were higher than that in the control, respectively（both P0.01）. The increased expression of hCLCA1mRNA in allergic rhinitis group were well correlated with the expression of MUC5ACmRNA and MUC5AC protein and the correlation coefficients were 0.752 and 0.694（both P0.05）.Conclusion：Upregulation of hCLCA1 mRNA expression may play a pivotal role in mucus overproduction in allergic rhinitis group.The inhibition of HCLCA1 expression may provide a new strategy for the treatment of allergic rhinitis.