甲氰菊酯在家兔体内的死后分布研究

Study on the postmortem distribution of fenpropathrin in rabbits

目的建立甲氰菊酯家兔灌胃染毒致死模型和生物检材中甲氰菊酯的气相色谱和气相色谱-质谱联用检测方法,研究甲氰菊酯在家兔体内的死后分布规律。方法家兔6只,甲氰菊酯经口灌胃染毒,死亡后迅速解剖,取心血、外周血、肝等组织,气相色谱和气相色谱-质谱联用法检测甲氰菊酯含量;部分组织经甲醛固定,HE染色,光镜观察其病理改变。结果家兔染毒后2～3h出现中毒表现,染毒后4.5～8h死亡。气相色谱和气相色谱-质谱联用法均检测到甲氰菊酯。甲氰菊酯在家兔体内死后分布为胃壁(458.92±32.82)μg/g、肾(46.47±6.30)μg/g、肝(35.79±20.11)μg/g、大脑(28.77±10.52)μg/g、心(26.49±4.10)μg/g、脾(22.23±5.37)μg/g、胆汁(10.87±1.42)μg/mL、肺(10.32±0.78)μg/g、周围血(8.14±1.12)μg/mL和心血(8.20±1.83)μg/mL。结论甲氰菊酯的灌胃染毒致死模型、气相色谱和气相色谱-质谱联用检测方法及死后分布规律可应用于甲氰菊酯中毒死亡案件的法医学鉴定及法医毒物动力学研究。

Objective To develop a rabbit model of fenpropathrin poisoning by intragastric administration,to establish a method for qualitative and quantitative analysis of fenpropathrin in organs and fluid in rabbits,and investigate the postmortem distribution of fenpropathrin in poisoning death rabbits.Methods Six rabbits died from fenpropathrin poisoning were dissected as soon as their vital signs disappeared after intoxication.The samples of heart blood,peripheral blood,bile,liver,kidney and so on were collected.Analysis was performed with a GC equipped with a NPD and a GC-MS.Microscopic examination was also conducted in HE-stained tissue sections.Results Poisoning signs were observed 2～3h after fenpropathrin delivery.Deaths occurred in all rabbits at 4.5～8h after intoxication.Fenpropathrin could be detected in all of the samples except for in urine by GC or GC-MS with highest concentration in the gastric wall （458.92±32.82）μg/g,followed by kidney （46.47±6.30）μg/g,liver （35.79±20.11）μg/g,brain（28.77±10.52）μg/g,heart （26.49±4.10）μg/g,spleen （22.23±5.37）μg/g,bile （10.87±1.42）μg/mL,lung （10.32±0.78）μg/g,peripheral blood （8.14±1.12）μg/mL and heart blood （8.20±1.83）μg/mL.Conclusion Fenpropathrin in biological specimens can be determined by GC equipped with a NPD or GC-MS.The poisoning death animal model,the analysis and the postmortem distribution data of fenpropathrin can be applied to the forensic identification of fenpropathrin poisoning death.