超顺磁性氧化铁纳米粒子和CM-DiI荧光双标记骨髓间充质干细胞

Superparamagnetic iron oxide and chlormethylbenzamido-1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine labeling and tracing bone marrow mesenchymal stem cells

背景:细胞移植疗效监测取决于有效的标记方法,从而能够对移植细胞进行示踪。超顺磁性氧化铁纳米粒子(superparamagnetic iron oxide,SPIO)是一种较为理想的新型磁共振对比剂,用SPIO标记细胞可实现对移植细胞进行活体示踪。而荧光活性染料CM-DiI无细胞毒性,不影响细胞的生长,适合标记和示踪细胞。目的:观察SPIO及CM-DiI对骨髓间充质干细胞的标记及示踪效果。方法:全骨髓法培养猪骨髓间充质干细胞,用含50mg/L铁浓度的SPIO及CM-DiI标记骨髓间充质干细胞,将双标后的骨髓间充质干细胞经冠状动脉注入猪心肌梗死模型。4周后取心脏组织行冰冻切片观察。结果与结论:SPIO及CM-DiI在体外标记骨髓间充质干细胞效率高,几乎达100%。经冠状动脉移植4周后心肌组织可找到双标记的骨髓间充质干细胞,结果提示SPIO及CM-DiI双标记骨髓间充质干细胞体内示踪效果好。

BACKGROUND：The success of cell therapy will depend on the ability to monitor the fate of transplanted cells in vivo.Superparamagnetic iron oxide（SPIO） labeling is an ideal magnetic resonance contrast medium,and it offers the potential for non-invasive tracking of implanted cells.The chlormethylbenzamido-1,1＇-dioctadecyl-3,3,3＇,3＇-tetramethylindocarbocyanine（CM-DiI） labeling does not have cytotoxicity,so it cannot influence cell growth.OBJECTIVE：To investigate the effects of SPIO and CM-DiI labeling and tracking on bone marrow mesenchymal stem cells（BMSCs）.METHODS：Porcine BMSCs were isolated and cultured by the whole bone marrow method.BMSCs were labeled with SPIO containing 50 mg/L Ferrum and CM-DiI.The labeled BMSCs were transplanted into porcine myocardial infarction model via intracoronary infusion.The frozen sections of the cardiac tissues were obtained after 4 weeks.RESULTS AND CONCLUSION：Efficiency of SPIO and CM-DiI labeling BMSCs was nearly 100%.The SPIO and CM-DiI labeled BMSCs could be found in the cardiac muscle tissues at 4 weeks after transplantation.SPIO and CM-DiI labeling BMSCs were efficiently tracked in vivo.