干细胞因子促进牙髓干细胞增殖与骨向分化能力

Stem cell factor promotes the proliferation and osteogenic differentiation of dental pulp stem cells

背景:干细胞因子作为对干细胞的增殖分化具有一定促进作用的因子,是否影响牙髓干细胞的生物学特性从而在牙齿再生中发挥重要作用尚不清楚。目的:观察干细胞因子对人牙髓干细胞增殖与骨向分化能力的影响。方法:采用酶消化法培养因正畸需要拔出的健康人牙的牙髓组织,接种于培养板,加入含1,10μmol/L干细胞因子的培养液,以正常培养液作为对照。MTT法检测细胞增殖,real-timePCR检测成骨相关基因骨唾液蛋白、骨钙素mRNA的表达,碱性磷酸酶试剂盒检测碱性磷酸酶活性。结果与结论:干细胞因子对牙髓干细胞的增殖具有促进作用,干细胞因子上调了骨唾液蛋白、骨钙素mRNA的表达,增强了牙髓干细胞的碱性磷酸酶活性,且具有随浓度增加促进作用增强的趋势。提示干细胞因子可以促进人牙髓干细胞的增殖及骨向分化能力。

BACKGROUND：Stem cell factor（SCF） serves as a factor having a promotion effect on proliferation and differentiation of stem cells.Whether SCF affects biological features of dental pulp stem cells（DPSCs）,and plays an important role in tooth regeneration remain unclear.OBJECTIVE：To observe SCF effects on the proliferation and osteogenic differentiation of human DPSCs.METHODS：Pulp tissues were removed from healthy human teeth extracted for orthodontic purposes.The pulp tissues were digested by collagenase and dispase.The sample was cultivated in culture flasks with medium contained 1,10 μmol/L SCF;the normal culture medium served as control.The cell proliferation ability was detected by MTT.The osteogenic gene bone sialoprotein and osteocalcin mRNA expression were examined by real-time PCR.Alkaline phosphatase activity was detected by alkaline phosphatase kit.RESULTS AND CONCLUSION：SCF could enhance the proliferation ability,upregulate bone sialoprotein and osteocalcin mRNA expression,and enhance the alkaline phosphatase activity,with the tendency of increased promotion with increased concentration.Results indicated that SCF could enhance the proliferation and osteogenic differentiation ability of human DPSCs.