室管膜前下区神经干细胞向神经元分化中骨形成蛋白2及DLX5的作用

Role of bone morphogenetic protein 2 and DLX5 in the differentiation of neural stem cells from anterior subventricular zone into neurons

背景:目前神经干细胞应用的最大障碍还在于分化的调控,而对干细胞分化调控机制尚知之甚少。室管膜前下区是目前公认的神经干细胞富集区之一。在此区的神经干细胞可以长距离迁移到嗅球而保持神经干细胞不分化,最后在嗅球分化为中间神经元。骨形成蛋白2及DLX5在室管膜前下区神经干细胞向嗅球迁移分化过程中起着重要作用。目的:研究室管膜前下区神经干细胞的构筑及迁移特点,以及目前国内外有关骨形成蛋白2及DLX5在此区神经干细胞迁移分化中的作用,希望对神经干细胞的分化调控机制有一定的认识。方法:由第一作者利用中文检索词"神经干细胞,室管膜前下区,骨形成蛋白2,DLX5"及英文检索词"neural stem cells,SVZa,BMP-2,DLX5",在PubMed数据库(http://www.ncbi.nlm.nih.gov/PubMed)、中国期刊全文数据库(www.cnki.net/index.htm)、万方数据库(http://www.wanfangdata.com.cn)中检索1997-01/2009-01关于骨形成蛋白2、DLX5在室管膜前下区神经干细胞向神经元分化中作用的文献。排除内容陈旧或重复文献。结果与结论:初检得到121篇文献,中文86篇,英文35篇。最后对符合标准的28篇文献作进一步的分析。结果提示,室管膜前下区神经干细胞具有其独特细胞构筑和长距离迁移的特点,骨形成蛋白2及DLX5在其迁移分化过程中起着重要作用,但其具体作用机制仍不清楚。

BACKGROUND：Regulation of neural stem cells differentiation hampers its application,and the regulation mechanism remains poorly understood.Anterior subventricular zone（SVZa） is one of the rich zones of neural stem cells.The neural stem cells at SVZa can immigrate for a long distance with undifferentiated states and finally differentiate into interneurons at the olfactory bulbs.Bone morphogenetic protein-2（BMP2） and DLX5 play a significant role in immigration and differentiation of neural stem cells at SVZa towards olfactory bulb.OBJECTIVE：To study the feature of construction and immigration of neural stem cells at SVZa,and to review the role of BMP2 and DLX5 in neural stem cells differentiation,in addition,to explore the regulation mechanisms of neural stem cells differentiation.METHODS：Literatures from PubMed database（http：//www.ncbi.nlm.nih.gov/PubMed）,CNKI database（www.cnki.net/index.htm） and WANFANG database（http：//www.wanfangdata.com.cn） published between January 1997 and January 2009 were searched with the key words of ＂neural stem cells,SVZa,BMP2,and DLX5＂.The repetitive and obsolete studies were excluded.RESULTS AND CONCLUSION：A total of 121 literatures were selected and primarily collected,including 86 Chinese literatures and 35 English literatures.Finally,according to standardization,28 literatures were further analyzed.Neural stem cells of SVZa have unique construction and can immigrate for a long distance.BMP2 and DLX5 play a significant role in neuronal immigration and differentiation of the NSCs of SVZa,but the detailed mechanism of BMP2 and DLX5 in SVZa is not clear.