加味补阳还五汤对兔髂动脉球囊损伤血管狭窄和氧化应激的影响

Effects of Jiawei Buyang Huanwu Decoction on vascular stenosis and oxidative stress after balloon injury of rabbit iliac artery

背景:近年研究证明,补阳还五汤具有扩张血管、改善微循环、抗炎、抗氧化应激和保护血管内皮细胞的功能,但其具体机制尚不清楚,尤其是对经皮腔内冠状动脉成形后再狭窄形成过程有何影响,目前很少见报道。目的:观察加味补阳还五汤对兔髂动脉球囊损伤后血管狭窄及氧化应激的影响。方法:将新西兰兔以随机抽签法分为对照组、模型组和药物组。对照组给予普通饲料,模型组、药物组给予高脂饮食。饲养2周后,模型组、药物组行髂动脉内膜剥脱术,对照组兔行假手术对照,药物组术后饲料中添加加味补阳还五汤药颗粒2mL/(kg·d),对照组及模型组喂食同前。4周后光镜观察兔髂动脉内膜的损伤情况,并检测血脂水平、血清超氧化物歧化酶活性和丙二醛水平的变化。结果与结论:对照组髂动脉内膜薄且结构完整,无动脉硬化斑块;模型组内膜增厚,管腔明显狭窄,可见明显动脉粥样硬化斑块;药物组动脉粥样硬化斑块厚度减小,管腔狭窄程度较轻。药物组兔血清总胆固醇、三酰甘油和低密度脂蛋白胆固醇、丙二醛水平明显低于模型组,而高密度脂蛋白胆固醇、血清超氧化物歧化酶水平明显高于模型组(P<0.05)。结果说明加味补阳还五汤具有较好的防止家兔球囊扩张损伤髂动脉所致的管腔狭窄及抗实验性动脉粥样硬化作用,其机制可能与其清除氧自由基抗氧化应激、调节脂质代谢等作用有关。

BACKGROUND：Modern pharmacological studies have confirmed that Buyang Huanwu Decoction could expand blood vessels,improve microcirculation,decrease oxidative stress,inflammation and platelet activation,so protect and improve the function of endothelial cells.But its certain mechanism is still unclear,especially for its impact on the restenosis of the coronary artery after percutaneous transluminal coronary angioplasty （PTCA）.OBJECTIVE：To investigate the protective effects of Jiawei Buyang Huanwu Decoction on vascular stenosis and oxidative stress after balloon injury of rabbit iliac artery.METHODS：New Zealand rabbits,were randomized and divided into three groups,control group,model group and drug group respectively.Rabbits of control group were fed with common forage,but model group and drug group fed with high fat diet.Two weeks later,the iliac arteries were injured by balloon for model group and drug group.Meanwhile drug group were fed with Jiawei Buyang Huanwu Decoction after the operation,2 mL/kg per day.Control group underwent sham operation control.At the end of 4 weeks,serum samples were stored to assay the levels of cholesterol,activity of superoxide dismutase （SOD） and levels of malondialdehyde （MDA）.Injured iliac artery was fixed by neutral formalin to observe the endothelial hyperplasia by light microscope,and the results were analyzed by picture analysis system.Differences of measurement were compared with one-way analysis of variance as well as repetitive measurements analysis of variance.RESULTS AND CONCLUSION：Iliac artery intima was thin,with intact structure,without arteriosclerosis in the control group.The vascular lumina were narrower,intima was thicker and there were more enormous arteriosclerosis plaques in model group rabbits.The plaque thickness was reduced and stenosis was mild in drug group.Total cholesterol,triglyceridemia,low-density lipoprotein of cholesterol （LDL-C） and MDA levels were significantly lower in the drug group compared with the model group,whereas high-density lipoprotein of cholesterol （HDL-C） and serum SOD levels were significantly greater than the model group （P 0.05）.The results showed that Jiawei Buyang Huanwu Decoction has significant preventive effect on intimal hyperplasia and the development of artherosclerosis in rabbits with iliac artery injury,and the mechanism of which may be related to modifying lipid metabolism and cleaning oxygen free radical anti-oxidative stress.