厌氧菌介导的CD/5-FC系统对鼠黑色素瘤的杀伤效应

The Killing Effect of CD/5-FC System Delivered by Anaerobic Bacteria on Melanoma Cell of Mice

目的构建婴儿双歧杆菌介导的CD/5-FC肿瘤基因治疗系统,探讨该系统对小鼠黑色素瘤细胞B16-F10的体外杀伤效应。方法构建重组质粒pGEX-CD,以电穿孔法转化婴儿双歧杆菌,筛选阳性克隆菌,然后加入5-FC厌氧培养,24h后取其上清,作用于小鼠黑色素瘤B16-F10细胞,采用MTT法检测细胞存活率,并观察细胞形态学改变。结果成功构建出婴儿双歧杆菌介导的CD/5-FC肿瘤基因治疗系统。与对照组和pGEX组相比,CD组的肿瘤细胞发生了明显的形态学改变,细胞存活率明显降低。结论婴儿双歧杆菌介导的CD/5-FC系统对小鼠黑色素瘤B16-F10细胞具有明显的体外杀伤效应。

ObjectiveTo construct CD/5-FC tumor gene therapy system delivered by bifidobacterium infantis, and to investigate the killing effect on melanoma B16-F10 cell of mice.MethodsRecombined plasmid pGEX-CD was constructed and transferred into bifidobacterium infants by electroporation. Positive recombined bacteria was selected and identified, and then was incubated with 5-FC in anaerobic condition. The supernatant of bacteria was collected and added to the melanoma B16-F10 cells. The cell growth status was evaluated by MTT assay, and the morphologic changes of cells were observed. ResultsThe CD/5-FC tumor gene therapy system delivered by bifidobacterium infantis has been constructed successfully. The survival rate of cells was significantly lower in CD group than those in control group and in pGEX group,and tumor cells of CD group were remarkably damaged morphologically. ConclusionThe CD/5-FC system delivered by bifidobacterium infantis has the significant killing effect on melanoma B16-F10 cell of mice in vitro.