甲状腺乳头状癌组织中TSHR基因启动子甲基化研究

Methylation of TSHR Gene Promoter in Papillary Thyroid Carcinoma

目的探讨甲状腺乳头状癌组织中促甲状腺激素受体基因(thyroid stimulating hormone receptor,TSHR)基因启动子区5′端CpE岛甲基化改变的特点与临床特征的关系。方法采用甲基化特异性PCR(methylation-specific PCR,MSP)方法检测TSHR基因启动子甲基化情况。结果 (1)甲状腺乳头状癌组织中TSHR基因启动子甲基化的发生率为64.7%(22/34),癌旁组织中TSHR基因启动子甲基化的发生率为26.5%(9/34),癌组织中TSHR基因启动子甲基化率显著高于癌旁组织(P<0.05)。(2)有淋巴结转移的甲状腺乳头状癌组织TSHR基因启动子甲基化的发生率为83.3%(15/18),高于无淋巴结转移组43.8%(7/16)(P<0.05)。结论 TSHR基因启动子异常甲基化是甲状腺乳头状癌发展过程中的分子事件之一,可能影响了甲状腺乳头状癌细胞的摄碘的功能。

ObjectiveTo investigate the relationship between the characteristics of promoter methylation of thyroid stimulating hormone receptor（TSHR） gene in papillary thyroid Carcinomas（PTC） and the clinical manifestation of PTC. MethodsThe methylation status of TSHR gene was detected by methylation specific PCR technique（MSP）.Results（1） The methylation rate of TSHR gene in PTC tissues was 64.7%（22/34）,while the methylation rate of TSHR gene in adjacent thyroid tissues（ATT） was 26.5%（9/34）, and the rate of methylation of TSHR promoter in PTC was significantly higher than of ATT（P〈0.05）. （2）The rate of methylation in PTC patients with lymph nodes metastasis was 83.3%（15/18）, which was significantly higher than that without lymph nodes metastasis 43.8% （7/16）.（P〈0.05）. ConclusionThe methylation of promoter in TSHR Gene is a common molecule event and may play a role in iodide-uptake of human PTC.