金属锌离子对胆绿素的荧光增强作用

Fluorescence Enhancement of Biliverdin by Zinc Ion

研究了不同条件下Ｚｎ２＋对胆绿素（ｂｉｌｉｖｅｒｄｉｎ，ＢＶ）荧光性质的影响作用。发现在无水乙醇、甲醇、ＮＨ３－ＮＨ４Ｃｌ缓冲体系和ＮａＯＨ水溶液中，Ｚｎ２＋均对ＢＶ有显著的荧光增强作用。在ＮａＯＨ水溶液中，在表面活性剂（ｃｅｔｙｌｔｒｉｍｅｔｈｙｌａｍｍｏｎｉｕｍｂｒｏｍｉｄｅ，ＣＴＡＢ）存在下，Ｚｎ２＋与ＢＶ的摩尔比率为２，荧光增强作用达到最大值。借助这一荧光增强技术，可以在室温下使胆绿素水溶液产生特征强荧光（λｅｘ＝４６５ｎｍ，λｅｍ＝５２５ｎｍ）。

The use of metal ions for the fluorescence enhancement of biliverdin in sever- al media was investigated. It was found that adding zinc salt increased fluorescent in- tensity of biliverdin remarkablely in methanol, ethanol, NH3 - NH4Cl buffer and aqueous alkaline solutions etc. The fluorescence enhancement reached its maximum when mole ratio of zinc ion to biliverdin was 2 in the presence of surfactant cetyltrimethyl ammonium bromide (CTAB) in aqueous alkaline solution . By means of the fluorescent enhancement, the specific strong fluorescence of biliverdin(λex= 465nm, λem= 525nm ) was obtained in aqueous solution at room temperature.