醌氧化还原酶1 C609T基因多态性与大肠癌关系

Relationship between NQO1 C609T gene polymorphism and risk of colorectal cancer

目的探讨NQO1基因C609T变异与大肠癌易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）基因分型技术,对268对大肠癌患者和对照者NQO1基因cDNA609位点多态性进行测定。结果病例组中基因型为C/C、C/T、T/T的分别有68,149,69例,分别占23.77%,52.10%和24.13%;对照组分别有116,126,44人,占40 56%,44.06%和15.38%;3种基因型频率在2组中分布差异均有统计学意义（P〈0.05）;以C/C基因型作为参照基因型,C/T和T/T2种基因型的调整OR分别为2.06（95%CI=1.37~3.10）和2.64（95%CI=1.57~4.44）;NQO1C609T变异基因型与吸烟、饮用水及食物类型存在交互作用而增加个体患大肠癌的危险。结论携带NQO1C609T,变异基因型个体患大肠癌的风险增加,且该变异基因型与环境因素具有协同致癌作用。

Objective To explore the relationship between NQO1 C609T gene polymorphism and the risk of colorectal cancer.Methods A hospital-based case-control study was conducted and polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method was used to detect the genotypes.Results The frequency of C/T,T/T genotype among cases with colorectal cancer were higher than those among the controls.As compared with the C/C genotypes,the NQO1 C/T,T/T genotype significantly increased the risk for developing colorectal cancer and the OR were 2.061（95%CI=1.372-3 097） and 2.640（95%CI=1.568-4.444）,respectively.37.41% of C and 62.59% of T allele were distributed in colorectal cases while 50.17% of C and 49.83% of T in controls.In addition NQO1 609 any T ganotype might interact with smoking,drinking water and types of food to increase the risk of colorectal cancer.Conclusion NQO1 609 any T genotype might increase the risk of colorectal cancer development and the genotype might interact with other environmental factors to increase the risk of colorectal cancer.