不溶性镍化合物对人肺癌A549细胞镍含量影响

Effect of water-insoluble nickle compounds on cellular nickle contents in human lung cancer A549 cells

目的探讨不溶性镍化合物对细胞中镍含量的影响。方法用不同浓度的水不溶性氧化镍(NiO)和二硫化三镍(Ni3S2)分别处理人肺癌A549细胞,用原子吸收分光光度计(AAS)检测染毒48 h后细胞内镍含量并估算其摄取率。结果经过终浓度为0,0.5,1.0,1.5,2.0,2.5,3μg/cm2的Ni3S2处理后,平均每105个细胞中含镍量为0.002 4,0.077 1,0.015 7,0.226 8,0.475 4,0.7,0.988 2μg;终浓度为0,0.5,1.0,2.0,4.0,8.0,16μg/cm2的NiO处理后,平均每105个细胞中含镍量为0.003,0.07,0.108,0.255,0.628,1.543,6.571μg;相同浓度(0.5,1.0,2.0μg/cm2)镍颗粒物处理下,Ni3S2处理组细胞镍含量分别为(0.077 1±0.006 6,0.157 4±0.019 0,0.475 4±0.112 1)μg/105个细胞,均高于NiO处理组(0.070 1±0.003 2),(0.108 1±0.015 9),(0.255 2±0.041 5)μg/105个细胞,各剂量组间比较差异均有统计学意义(P<0.05)。结论人肺癌A549细胞对不溶性镍化合物的摄取与其染毒剂量成正比,且相同浓度下对NiS的摄取率高于NiO。

Objective To investigate the effect of water-insouble nickle（Ni） compounds on cellular Ni contents.Methods After 48 hours＇ exposure of various concentrations of water-insoluble NiO and Ni3S2,the cellular Ni concentration in human lung cancer A549 cells was detected by atomic absorption spectrophotometer（AAS） to estimate the uptake rate.Results After treated with concentrations of 0,0.5,1.0,1.5,2.0,2.5,3 μg/cm^2 of Ni3S2,the Ni concentrations in every 10~5 cells were 0.0024,0.0771,0.01574,0.2268,0.4754,0.7,and 0.9882 μg,respectively.At the concentations of 0,0.5,1.0,2.0,4.0,8.0,and 16 μg/cm^2 of NiO,the Ni concentrations in every 10~5 cells were 0.003,0.07,0.108,0.255,0.628,1.543,and 6.571μg,respectively.At the same concentraions（0.5,1.0,2.0μg/cm^2）,Ni concentrations of the cells treated with Ni3S2 were 0.0771±0.0066,0.1574±0.0190,and 0.4754±0.1121,and higher than that of NiO treat-ed cells（0.0701±0.0032,0.1081±0.0159,0.2552±0.0415） with significant difference between the two groups（P0.05）.Conclusion The uptake of water-insouble Ni compound is dose-dependent and the uptake rate of Ni3S2 is higher than that of NiO at the same Ni concentration.