摘要
目的探讨在颗粒脂肪中加入脂肪来源的间充质干细胞(ASCs)后,能取得更好移植效果的可能性。方法以兔为模型,在一侧腹股沟处取出脂肪组织并剪成颗粒状,分成二等份,每份0.4g;取出另一侧脂肪组织,0.25%胶原蛋白酶消化,过筛离心,收集离心后沉淀物中的组织0.2g,加入其中的一份颗粒脂肪中为实验组,另一份为对照组,两份组织分别植入兔背中线的两侧,3个月后取出称重计算成活率,进行两组之间的比较。结果颗粒脂肪加入ASCs后,与对照组比较,移植后成活率增多,差异有统计学意义(P<0.05)。结论ASCs加入颗粒脂肪组织中进行游离移植,可取得更好的移植效果,将成为临床脂肪移植提供一个新方法。
Objective To investigate the possibility to achieve a better result of fat particles combined with adipose-derived stem cells（ASCs）. Methods Fat tissue was harvested in the inguinal regions of a rabbit.One side fat tissue was cut into granules and divided into two even parts（0.4g per part）;the other side fat tissue was sifted and centrifuged after 0.25% collagenase digestion.Precipitation was harvested and 0.2g precipitation was mixed with one part of fat granules,which was the experimental group,and the other part granules was the control group.The tissue in the experimental and control group was randomly implanted into two sides near the median dorsal line of a rabbit.The grafts were taken out and weighted after 3 months,and the survival rates were calculated and the differences between two groups were compared. Results The survival rate increased remarkably in the experimental group,compared with that in the control group,and the differences were statistical significant（P〈0.05）. Conclusion A better transplantation effect could be obtained implanting the fat particles mixed with ASCs,which is suggested to have the potential to become a new method of fat graft in clinical practice.
出处
《健康研究》
CAS
2009年第6期419-421,437,共4页
Health Research
基金
浙江省科技厅科研基金(No.2009476)
