加速溶剂萃取—液相色谱—紫外检测法测定人参中多种人参皂甙含量

Determination of Ginsenosides in Ginseng by ASE-SPE-LC-UV method

人参中多种人参皂甙含量测定,采用加速溶剂萃取(ASE)技术制备样品溶液,用安装有Acclaim 120 C18(250mm×4.6mm,5μm)的色谱柱及紫外检测器(UV)的液相色谱仪测定。对6种主要人参皂甙Re、Rg1、Rf、Rb1、Rc、Rb2进行分析,6种人参皂甙在2.5～80mg/mL范围内线性良好,相关系数均大于0.9986,最低检测限为25.0～50.0mg/kg,样品添加回收率为85.69～102.79%,相对标准偏差(RSD%)为0.50～1.283%.

The Ginsengnosides in ginseng were determinted by High Performance Liquid Chromatography equipped with a column of Acclaim 120 C18（250mm×（4.6mm, 5μm） and the Detector（UV）.The ginseng samples were extracted by Acclerated Solvent Extraction（ASE）o The good linearities were obtained for 6 main ginsengnoside Re, Rg1, Rf, Rb1, Rc, Rb2 in the range of 2.5 - 80mg/mL.The correlation coefficient of standard curves wae more than 0.9986.The limits of detection from 25.0 to 50.0mg/kg.The average recoveries for those ginsengnoside in ginseng were from 85.6 to 102.79% ,the relative standard deviations were 0.50- 1.283%.