重组人骨形成蛋白-2和牛骨形成蛋白骨诱导活性的比较研究

A Comparative Study on Osteoinductive Activities of Recombinant Human BMP 2 and Bovine BMP

将５００μｇ重组人骨形成蛋白－２（ｒｈＢＭＰ－２）和５００μｇ牛骨形成蛋白（ｂＢＭＰ）以胶原作为载体分别植入同一ＳＤ大鼠两侧股部肌肉内。取材后进行组织学观察和钙含量测定。结果显示：术后１周，两组植入局部均可见软骨细胞分化和软骨形成，胶原大部分被降解吸收；３周，两组均诱导板层骨和骨髓形成，胶原被完全降解吸收；ｒｈＢＭＰ－２组诱导形成的软骨或骨组织的量明显多于ｂＢＭＰ组。此结果提示：ｒｈＢＭＰ－２的骨诱导活性优于ｂＢＭＰ。

To compare the osteoinductive activities of recombinant human bone morphogenetic protein 2(rhBMP 2)and bovine bone morphogenetic protein (bBMP),500 μg rhBMP 2 and 500 μg bBMP were implanted into the left and right calf muscle pouch of SD rats using collagen as a carrier. Histologic examination showed cartilage was induced in both groups 1 week later ,and the collagen was absorbed partilly. Bone and bone marrow were induced in both groups 3 weeks later, and the collagen was absorbed completely. rhBMP 2 induced more cartilage or bone than bBMP did. Calcium content of rhBMP 2 implanted region was higher than that of bBMP implanted region. It suggests that ectopic osteoinductive activity of rhBMP 2 is better than that of bBMP.