鸡红细胞体外融合最适条件的研究

Optimum Conditions for in vitro Fusion of Chicken's Red Cells

探讨快速、有效的细胞融合条件,以一龄公鸡红细胞为材料,聚乙二醇(MW=1500)为诱导剂,研究不同的细胞密度、不同温度、融合时间、CaCl2浓度等因素对细胞融合率的影响。结果显示:细胞密度为1.0×107/mL,39℃,与PEG作用10 min,CaCl2浓度为50 mmoL/mL时融合效果最好。

How to rapidly and effectively fuse cells was studied.By taking red cells of a one-year old rooster as materials,polyethylene glycol（MW= 1 500） as the revulsant,the authors studied the effect of cell density,temperature,fusion time,CaCl2 concentration,etc.on cell fusion.The results showed that the best fusion was gotten when cell density was 1.0×10^7/mL,temperature was 39 ℃,and the PEG role time was 10 min,CaCl2 concentration was 50 mmoL/ml.