摘要
利用分子生物学实验手段针对陕西分离株的VP2基因进行原核表达。根据IBDV全基因序列中VP2的编码区设计一对引物，以IBDV陕西分离株的cDNA作为模板，采用PCR方法扩增出VP2基因，构建重组质粒。利用PCR方法能够扩增出大小为1500bp的目的片段，酶切鉴定后做SDS-PAGE电泳明显观察到有一条60ku的蛋白条带。表明VP2基因获得表达。该研究为进一步研究IBDV陕西分离株氨基酸序列变化及抗VP2的单克隆抗体的制备提供了技术支持。
The method of molecular biology was used to express VP2 Gene of IBDV Shaanxi isolated strain in E. coli. One pair of primers was designed by the reign of VP2 gene in IBDV genome. DNA of IBDV Shaanxi isolated strain was used as templates for PCR. The productions of CR were cloned into plasmid. Results showed that a 1500bp fragment was obtained in PCR. And a 60ku protein was ob- served in SDS-PAGE electrophoresis. It showed the VP2 gene of IBDV Shaanxi isolated strain was currently expressed. This research will provide technical support for the production of monoclonal an- tibodies and further researches of alteratio;1 of amino acid sequence in IBDV SHAANXI isolated strain.
出处
《西安职业技术学院学报》
2008年第1期10-15,共6页
Research on Vocational Education in Xi'an Vocational and Technical College
