抽提条件对饲料酶制剂中木聚糖酶活力的影响

Effect of Extracted Conditions on Xylanase Activity in Feed Enzyme Preparation

用蒸馏水、0．1mol／L氯化钠、0．02mol／L（pH7．4）的磷酸缓7中液，分别于4℃、40℃对浙江酶、华芬酶、Avizyme-1500三种酶制剂抽提12小时和1．5小时，对抽提到的酶液进行木聚糖酶活力测定的结果表明，用0．1mol／L的氯化钠溶液于4℃抽提12小时对华芬酶、Avizyme-1500酶制剂中的木聚糖酶的抽提效果最佳；对浙江酶而言，是蒸馏水（4℃，12小时）的抽提效果最好。

Xylanase activity was extracted from Zhejiang, Huafen and Avizyme - 1500 enzyme preparation at 4℃ and 40℃ for 12h and 1.5h by distilled water, 0. 1mol/L NaCI, 0.02 mol/L（pH 7.4） sodium phosphate buffer. The result showed that the xylanase activity extracted by 0. 1 mol/L NaCl from Huafen and Avizyme - 1500 enzyme preparation at 4℃for 12h was best. Meanwhile xylanase activity was the highest in Zhejiang enzyme preparation when distilled water was used as extrction solution at 4℃ for 12h.